A preliminary study on RAPD analysis protocol in potato
LI Xian ping,HE Yun kun,SUN Mao ling,LI Yun hai,DEN Ji xing,ZHAO Zhi jian (Biotechnology Research Institute of YAAS,Kunming 650223,China)
The objective of the present study was to analyse the potato RAPD protocol by using the main potato cultivars of Yunnan province.The results indicated that the optimum RAPD conditions for potatoes were:template concentration ranging 15 50ng;10 mer random primer 25 50ng;dNTP concentration 200μm;denaturing at 94℃ for 1 min,annealing at 37℃ for 1 min,extension at 72℃ for 2 min and the number of cycles from 40 to 45,All of the 11 cultivars were clearly identified by RAPD.The results indicated that the genetic background of cv. CFK69 1 was very special,its genetic distance was far from other cultivars.
【CateGory Index】： S532.03