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《Southwest China Journal of Agricultural Sciences》 2008-01
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PCR detection of Nematode isolated from Panax notoginseng

FENG Guang-quan1,DONG Li-ying2,CHEN Yu-jun1,SHANG Hui2,LIU Yun-zhi1,LI Jia-rui2,YANG Jian-zhong1,CUI Xiu-ming1,YANG Pei-wen2* (1.Wenshan Prefecture Sanqi Science and Technology Research Institute,Yunnan Wenshan 663000,China;2.Institute of Agricultual Environment and Resource,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China)  
Based on the genomic rDNA sequence,a pair of oligonucleotide primer which was specific for M.hapla.was designed.DNA of nematodes extracted from Wenshan population,Yanshan population,Maguan population,Mengzi populagtion and other 4 Meloidogyne spp.populations(M.hapla,M.incognita,M.arenaria and M.javanica) were amplified with the primers.The results showed that about 462bp fragments were amplified from genomic DNA of Wenshan population,Yanshan population,Maguan population,Mengzi populagtion and M.hapla,but other Meloidogyne spp.(M.incognita,M.arenaria and M.javanica) populations had no amplification products.It indicated that Wenshan population,Yanshan population,Maguan population and Mengzi populagtion were M.hapla.The pair of primers would be useful for molecular identification and detection of root-knot nematode in P.notoginseng.
【Fund】: 云南省科技攻关项目(2005NG07)
【CateGory Index】: S435.672
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