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《Southwest China Journal of Agricultural Sciences》 2011-01
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Preliminary Study on Response Rate of Waxy Corn and Quality Protein Maize to Anther Culture

HE Xue-yin1,WU Cui-rong1,TIAN Shu-yun1,SU Yue-gui1,QIN Lan-qiu1,WU Yong-sheng1,WEN Ren-lai1,XIE Chuan-xiao2(1.Maize Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530227,China;2.Crop Science Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)  
The response rate of 14 waxy corn landraces and 10 quality protein maize cultivars to anther culture in vitro was studied with basic medium 82-1.The results showed that 13 out of the 14 waxy corn landraces tested had response to anther culture in vitro accounted for 92.86 % and the highest response was Zuo-deng-nuo with the generation of embryo-like structures being 3.16 per 100 anthers;5 out of 10 quality protein maize(QPM) collections cultured responsive to anther culture in vitro accounted for 50.00 % and the highest response was CML170 with the generation of embryo-like structures being 0.65 per 100 anthers.3 out of those 13 waxy corn landraces responsive to anther culture in vitro can regenerate the normal plantlets with roots and buds from the embryo-like structures,with the regeneration of the normal plantlets of the waxy corn Zuo-deng-nuo,Liang-qiang-nuo,Tian-e-nuo being 4.08 %,9.09 %,25.00 % respectively.1 out of those QPM collections responding namely CML170 can reproduce the normal plantlets with roots and buds from the embryo-like structure,with the regeneration of the normal plantlets being 10.00 %.The results also indicated that waxy corn landrace had higher responsiveness than QPM in the production of embryo-like structure and the regeneration of the green plantlets and the response of waxy corn or QPM to anther culture in vitro existed genotype-dependent as normal maize.
【Fund】: 广西科学基金项目(桂科青0991073);; 广西农业科学院科技发展基金项目(2006009);; 转基因生物新品种培育重大专项项目(200908010-008B)
【CateGory Index】: S513
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