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《Southwest China Journal of Agricultural Sciences》 2011-01
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Research of Isolating Low-temperature-induced Protein from Tea Seedling by Different Extracting Methods

ZHANG Nan,HONG Yong-cong,DING Zhao-tang,WANG Yu(Tea Research Institute of Qingdao Agricultural University,Shandong Qingdao 266109,China)  
The effects of low temperature on cell memberane penetrability and soluble protein content in tea seedling leaves were studied.Different methods were used to extract and isolate low-temperature-induced proteins from tea seedling leaves after cold acclimation treatment.The results showed that tea seedling leaves treated by cold acclimation was propitious to induce low-temperature-induced proteins compared with the abscised leaves.Cell memberane penetrability of those seedling leaves were tested to have a tiny change,and the soluble protein content maintained a certain level.Compared with acetone precipitation method,TCA/ acetone precipitation method,ammonium sulphate precipitation method and direct extraction method,improved Tris-HCl extraction methods was the optimum method for extracting low-temperature-induced proteins in tea seedling leaves.This method showed the characteristics of high protein yields,clear protein band,few impurity disturbance and easy operation.In addition,in the SDS-PAGE electrophoretic pattern,three new protein bands(25,35,90 KDa) of the extraction samples appeared.
【Fund】: 青岛市科技计划项目(07-2-3-6-jch);; 山东省自然科学基金(2009ZRB01654)
【CateGory Index】: S571.1
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