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《Southwest China Journal of Agricultural Sciences》 2011-01
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Key Technology and Procedure of Industrial Seedlings on Sweet Potato for Fuel Ethanol——Large Scale Indoor Rapid Propagations

QIN Ting-hao1,ZHANG Jun1,ZHANG Yun1,YANG Hai-ping2(1.Sichuan Academy of Botany Engineering,Sichuan Zizhong 641200,China;2.Neijiang Agriculture Sicence and Technology Limited Company,Sichuan Neijiang 641000,China;3.ZiZhong Technology Bureau,Sichuan Zizhong 641200,China)  
The optimum indoor culture conditions for industrial seedlings have been established through the industrial seedling research of key technologies and procedures on the sweet potato for fuel ethanol.The anisothermal heat treatment was conducted on germinative sweet potatoes and aseptic seedlings respectively.Combined with biotechnology,the sweet potato stem-apex with 1 or 2 leaf primordiums was peeled off;after two weeks induction culture in the MS+BA0.5+NAA0.1 medium,the stem-apex was transfered into the MS+BA0.5+NAA0.1GA1.0 medium for plantlet generation.Then,the regenerated plantlet line was divided into three parts,each part was used for the Nitrocellulose Membrane Enzyme-Linked Immunosorbnent Assay(NCM-ELISA),Indicator Plant Test and preservation of rapid propagations,respectively.After the detection,the excellent plant lines was selected and used for industrial production,the results showed that the proliferation multiple could reach up to 5.9 at 27±1℃ in the MS0 medium within 25 days,and the transplants survival rate was more than 95% in the substrates with river sand,perlite and mixed humus soil.
【Fund】: 国家科技支撑计划项目(2007BAD78D00)
【CateGory Index】: S531
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