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《Southwest China Journal of Agricultural Sciences》 2011-01
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Constructing Fusants by Protoplast Fusion between Oryza and Eleusine Pathotypes of Magnaporthe oryzae

YIN Liang-fen1,2(1.College of Plant Science and Technology,Huazhong Agricultural University,Hubei Wuhan 430070,China;2.Key Lab of Crop Disease Monitoring and Safety Control in Hubei,Huazhong Agricultural University,Hubei Wuhan 430070,China)  
The genetic analysis of important genes,e.g.avirulence genes,antibiotic resistant genes in Magnaporthe oryzae pathogens had been limited because of the difficulty of getting highly fertile strains for genetic cross,especially in Oryza pathotype strains.In the present study,an alternative method for the genetic study of this fungus was tried by using the protoplast fusion system.Firstly,drug-resistant transformants were generated from the Oryza strain Y93-164a-1 and Eleusine pathotype strain SA98-4,then protoplasts of both selected transformants were fused by protoplast fusion technique.Stable fusants were selected after at least five subcultures and three single-spored isolations on the selective medium.Results suggested that the stable fusants can be easily obtained by protoplast fusion system,showing the protoplast fusion system could be usable for genetic analysis of M.oryzae pathogens.
【Fund】: supported by Program for New Century Excellent Talents in University(NCET-08-0783);; supported by a project (11660050) from the Ministry of Education Culture Sports Science and Technology of Japan;; the project of the Scientific Research Foundation for the Returned Overseas Chinese Scholars Huazhong Agricultural University China(4002-30541).
【CateGory Index】: S432.4
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