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《Southwest China Journal of Agricultural Sciences》 2011-01
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Cryopreservation of Dianthus caryophyllus Linn Germplasm by Vitrification

ZHOU Xu-hong1,HE Yan1,OUYANG De-ai2,GUI Ming1,LI Shen-chong1,CAO Hua1,YANG Chun-mei1,LU Zhen-hong1,MO Xi-jun1(1.Flower Research Institute of Yunnan Academy of Agricultural Sciences,Key Laboratory of Flower Breeding of Yunnan Province,Flower Engineering Center of Yunnan Province,Yunnan Kunming 650205,China;2.College of Life Science and Chemistry,Dali University,Yunnan Dali 671000,China)  
Shoot tips from Dianthus caryophyllus Linn cultivars 'YunLianDie',were successfully cryopreserved by vitrification method.Orthogonal design was used to analyze the effects of dominant parameters on survival rates of shoot tips,including sucrose concentration of preculture medium,duration of preculture,duration of loading and duration of exposure to PVS2.The result showed that the highest shoot tip survival rate was: preculture 1 days,0.5 mol/L sucrose,loading time 40 minutes,PVS2 treatment in 40 minutes,liquid nitrogen treated 1d and throwing in 40 ℃ water,the survival rate was up to 44.13 %.The regenerated plantlets showed no more difference than the normal plantlets in physiological and biochemical characters.This test set up successfully a cryopresevation by vitrification system technique for Dianthus caryophyllus Linn germplasm,which provided an effective way for long-lasting conservation in vitro of Dianthus caryophyllus Linn germplasm resources.
【Fund】: 云南省星火产业带项目(2006NG37)
【CateGory Index】: S682.19
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