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《Southwest China Journal of Agricultural Sciences》 2011-01
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Screening of Bacterial Strains for Degradation of Lubricating Oil and Their Analysis for Presence of Alkane Hydroxylase Gene (alkB) by PCR

YU Ying1,GUO Zhi-long1,WEI Ze-feng1,LUO Wei1,HUANG Wei-jiu2,CHENG Bo-shui(1.School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China;2.School of Material Science and Engineering,Chongqing University of Technology,Chongqing 400054,China;3.Department of Applied Engineering of Petroproducts,Logistical Engineering University,Chongqing 401311,China)  
Lubricating oil degrading bacteria were isolated from polluted acidic soil in Chongqing.Their taxonomic status were investigated by morphology observation and alignment analysis of 16s rDNA.The isolated bacteria's growing characteristics were tested when growing on lubricating oil as the sole carbon source.Their alkane hydroxylase gene(alkB) were analyzed by PCR.As a result,11 strains of bacteria were isolated.By enzymatic assay and the dry weight assay,9 strains were found to have ability to degradate lubricating oil.16srDNA analysis showed that these strains respectively belonged to Pseudomonas,Cupriavidus,Bacillus,Acinetobacter,Stenotrophomonas.PCR analysis suggested the alkB gene in isolated bacteria had different fragment sizes with the range between 160 and 400 bp.
【Fund】: 国家自然基金项目(50975282);; 重庆市杰出青年科学基金(CSTC 2008BA4037)
【CateGory Index】: X172
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