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《Southwest China Journal of Agricultural Sciences》 2012-01
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Preliminary Study on Callus Induction and Plant Regeneration of Rosa chinensis 'Slater's crimson China'

YAN Hui-jun1.2,ZHANG Hao1.2,JIAN Hong-ying1.2,WANG Qi-gang1.2,QIU Xian-qin1.2, ZHANG Ting1.2,TANG Kai-xue1.2 (1.Flower Research Institute of Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China; 2.Yunnan Flower Breeding Key Lab.,Yunnan Kunming 650205,China)  
The effects of different concentrations of 2,4-D,TDZ,GA3,NAA,combination with the condition of illumination on the status of embryogenic callus induced from young leaves of R.chinensis 'Slater's crimson China'were studied and the embryogenesis and plant regeneration of embryogenic callus were also investigated.The result showed that callus could be induced by the media MS+2,4-D 5mg/L + proline 300 mg/L.The best media for embryogenic callus induction was MS+TDZ 1.0 mg/L+GA3 0.1 mg/L+NAA 0.01 mg/L for 12 dark days.Cotyledonary embryoid and tissues structure of adventitious buds and embryonic root were obtained from embryogenic callus cultured under light for 15 days,and planted young plants for 20 days.
【Fund】: 国家自然科学基金项目(31060268);; 云南省青年基金项目(2010CD097);; 云南省科技创新强省计划项目(2007C0003Z)
【CateGory Index】: S685.12
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