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《Southwest China Journal of Agricultural Sciences》 2012-01
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Genetic Polymorphism of KAP 6.1Gene in Sheep

FENG Jing1,2,3,SONG Tian-Zeng2,3,YANG Hua2,YANG Jian-bo2,HAN Meng-li2,YANG Yong-lin2,SHI Guo-qing2, XU Cong-xiang2,HUANG Xi-xia1 (1.College of Animal Science,Xinjiang Agricultural University,Xinjiang Urumqi 830052,China;2.Breed & Biotechnology Key Laboratory of Sheep in Bintuan,Xinjiang Shihezi 832000,China;3.Institute of Animal Science,Tibet Autonomous Prefecture Academy of Agricultural and Animal Husbandry Science,Tibet Lhasa 626000,China)  
KAP6.1gene belongs to keratin-associated proteins(KAP) family,the wool fibre is a complex structure composed primarily of proteins from KAP gene family.These proteins are responsible for the major structural and mechanical properties of the wool fibre.PCR,SSCP and sequencing analysis were used to identify polymorphisms of KAP6.1gene in Chinese Merino(Xinjiang Junken Type).The results showed KAP6.1gene had C159T mutant site and 57bp insert/delete site.Three genotypes of C159T site existed in Chinese Merino(Xinjiang Junken Type).AA genotype frequency was 0.3694,AB genotype frequency was 0.1732,BB genotype frequency was 0.4574.A allele frequency was 0.4560,B allele frequency was 0.5440.Two genotypes of 57bp insert/delete site existed in Chinese Merino(Xinjiang Junken Type).AA(303 bp)genotype frequency was 0.9292,AB(303bp/246bp)genotype frequency was 0.0707.A allele frequency was 0.9646,B allele frequency was 0.0354.The results showed KAP6.1 gene could be candidate gene to study wool quality of merino sheep.
【Fund】: 转基因生物新品种培育科技重大专项(2009ZX08009-160B);; 新疆农垦科学院科技引导计划(YYD2010-8)
【CateGory Index】: S826
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