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Cloning and Expressing Analysis of Transcription Factor GhWRKY70 in Upland Cotton

LIU Dong-mei;LOU Xi-yan;YU Yong;LI Yao;PEI Dong-li;Key Laboratory of Plant-Microbe Interactions, Department of Life and Food Science, Shangqiu Normal University;College of Civil Engineering, Shangqiu Institute of Technology;  
【Objective】Cotton is one of the most important economic crops. The creation of male sterile materials and the mining of infertility-related genes has been a hot topic in cotton breeding. The cloning and expressing analysis of Gh WRKY70 gene laid the foundation for the study of the gene function research.【Method】Primer 5 was used in designing primers of Gh WRKY70 gene based on the transcriptome sequencing results,q-PCR verified the difference in expression of Gh WRKY70 gene in infertile and fertile anthers.【Result】The full-legth cDNA was cloned with a length of 918 bp, named Gh WRKY70, and Gene Bank No. is MF278614. The protein encoded by this gene contains a typical WRKY domain, and the C-terminus of each WRKY domain contains a C2HC-type zinc finger protein structure. 【Conclusion】Homology analysis shows that it belongs to the class III WRKY protein, and has the closest genetic relationship to Asian cotton and cocoa WRKY70. The upland cotton transcription factor Gh WRKY70 was differentially expressed in sterile anthers and fertile anthers of Dong A, suggesting that this gene may be associated with male sterility.
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