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Lu Huafei; He Yunkun; Zhang Zhongkai(Biotechnology Research Institute, YAAS Kunming 650223)Zhao Shuzhen; Wang Suyan; Ye Bin; Tian Bo(Institute of Microbiology, Chinese Academy of Sciences Beijing 100080)  
Yunnan commerclal tobacco cultivar G-28 and K326 were transformed by two strains of Agrobacterium tumefaciens with the plant expression vector pEC23 and pRCPⅠ respectively.A large number of transgenic plants were obtained.The plasmid vcctor pEC23 carried a marker gene expressing Km resistance and the gene of tobacco mosaic virus 54KD protein, the latter controlled by CaMV35s promoter. The plasmid vector PRCPⅠ carried the marker gene expressing Km resistance and two other genes, cucumber mosaic virus(CMV) coat protein and CMV satellite RNA, both the latter were controlled by CaMV35s promoter. The leaf disks of cultivar G-28 and K326 were transferred separately on MS medium added 0.2 mg/l NAA, 2.0mg/l 6-BA,0.1mg/l KT and 60mg/l Km for G-28, 20mg/l for K326 after cocultivation with A, tumefaciens with the plasmid vectors. Shoots formed from transformed leaf disks in 2 or 3 weeks. Then the shoots were excised off and in MS medium added 0.2mg/l NAA 60mg/l Km for G-28 or 20mg/l for K326 to further select the Km resistant shoots and stimulate to form roots. Finally, the Km resistance of rooted plants was detected. The results indicated that the genes, at least the Km resistant marker gene were introduced into the plants.
【Fund】: 云南省烟草公司
【CateGory Index】: S572.03
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