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《SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES》 1994-01
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ANTIVIRUS GENETIC TRANSFORMATION OF COMMERCUL TOBACCO CULTIVAR G-28 AND K326

Lu Huafei; He Yunkun; Zhang Zhongkai(Biotechnology Research Institute, YAAS Kunming 650223)Zhao Shuzhen; Wang Suyan; Ye Bin; Tian Bo(Institute of Microbiology, Chinese Academy of Sciences Beijing 100080)  
Yunnan commerclal tobacco cultivar G-28 and K326 were transformed by two strains of Agrobacterium tumefaciens with the plant expression vector pEC23 and pRCPⅠ respectively.A large number of transgenic plants were obtained.The plasmid vcctor pEC23 carried a marker gene expressing Km resistance and the gene of tobacco mosaic virus 54KD protein, the latter controlled by CaMV35s promoter. The plasmid vector PRCPⅠ carried the marker gene expressing Km resistance and two other genes, cucumber mosaic virus(CMV) coat protein and CMV satellite RNA, both the latter were controlled by CaMV35s promoter. The leaf disks of cultivar G-28 and K326 were transferred separately on MS medium added 0.2 mg/l NAA, 2.0mg/l 6-BA,0.1mg/l KT and 60mg/l Km for G-28, 20mg/l for K326 after cocultivation with A, tumefaciens with the plasmid vectors. Shoots formed from transformed leaf disks in 2 or 3 weeks. Then the shoots were excised off and in MS medium added 0.2mg/l NAA 60mg/l Km for G-28 or 20mg/l for K326 to further select the Km resistant shoots and stimulate to form roots. Finally, the Km resistance of rooted plants was detected. The results indicated that the genes, at least the Km resistant marker gene were introduced into the plants.
【Fund】: 云南省烟草公司
【CateGory Index】: S572.03
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【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 Wang Xihua 1 Zhang Feng 2 Zhao Zuntian 3 (1 Weifang Education Collage, Qingzhou 262500; 2 Jinan Education Collage, Jinan 250001; 3 Shandong Normal University, Jinan 250014);The Development of Study on Transgenic Plant in China[J];SHANDONG SCIENCE;1999-02
2 Liu Yule Wang Zhendong Zhu Feng Kang Liangyi Tien Po (Institute of Microbiology, Academia Sinica, Beijing 100080);TOLERANCE OF THE TRANSGENIC TOBACCO EXPRESSING SATELLITE RNA OF CUCUMBER MOSAIC VIRUS TO TOBACCO MOSAIC VIRUS[J];Acta Microbiologica Sinica;1994-05
3 ;Recent Advances of Tobacco Anti-virus Gene Technology[J];TOBACCO SCIENCE & TECHNOLOGY;2000-06
4 DONG ZHI-JIAN, ZHENG XIN-ZHANG,and LIU LI-QUANChina Tobacco Science and Technology Information Center, Zhengzhou 450000, China;Recent Advance in Environment-friendly Integrated Control of Tobacco Diseases and Pests[J];Tobacco Science & Technology;2002-12
5 Ye Changming Fan Huaizhong(Plant Protection Department, South China Agriculture University, GuangzhDu 510642)Ye Yin Tian Bo(Microbiology Institute, Academia Sinica, Beijing 100080);Sequencing and Construction of Plant Expression Vector of Coat Protein Gene from Ys Strain of Papaya Ringspot Virus[J];VIROLOGICA SINICA;1997-01
6 Yan Xiaolan; Qiu Guohua; Li Baojian(Biotechnology Research Center,Zhongshan University, Guangzhou510275);Directed Duplication of CaMV35S Promoter Influerues the Expression of the Foreign Genes in Transgenic Plants[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1995-02
7 Li Sheng-guo, Liu Yu-le , Zhu Feng, Luo Yu-ying, Kang Liang-yi and Tian Bo (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080);GENETICAL ENGINEERED MALE STERILE TOBACCO PLANTS AND THEIR SENSITIVITY TO TEMPERATURE[J];植物学报(英文版);1997-03
8 Zhou Ping; Pan Nai-sui Liu Chun-qing; Chen Zhang一liang(Nattonal Laboratory of Protein Engineering and Plant Gene:Engineering.of Peking Universiity,Beijing l00871);TRANSFER OF SWEET-RASTING PROTEIN THAUMATIN II GENE INTO TOBACCO USING TI-PLASMID VECTOR[J];CHINESE BULLETIN OF BOTANY;1994-04
9 Chen Yuxin 1 , Gao Yulong 2 , Ding Can 1 , Xu Zhaoli 2 ( 1 Agronomy College of Yunnan Agricultural University , Kunming 625014; 2 Yunnan Tobacco Science Research Institute , Yuxi Yunnan 653100);Construction of TMV and CMV Binary Virus Resistant RNA Silencing Vector[J];Chinese Agricultural Science Bulletin;2011-05
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