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《Journal of Southwest China Normal University(Natural Science Edition)》 2006-06
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Enzyme-Free Amperometric Hydrogen Peroxide Biosensor Based on Electro-Polymerization of Bromophenol Blue

FU Xiao-hong~(1,2),YUAN Ruo~1,CHAI Ya-qin~1,ZHANG Lin-yang~1,CHEN Shi-hong~1,TANG Ming-yu~11.School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China;2.College of Chemistry and Chemical Engineering,Yibin University,Yibin Sichuan 644007,Chin  
A novel enzyme-free amperometric hydrogen peroxide biosensor was developed by means of electro-polymerization bromophenol blue(PBPB) onto a platinum wire electrode.Experimental results showed that the PBPB-modified biosensor exhibited directly electrocatalytic behavior to the reduction of H_2O_2 without enzyme.The performance and factors influencing the proposed biosensor were studied in detail.Under the optimal conditions,the resulted electrode showed a linear response to hydrogen peroxide concentration ranging from 5.6×10~(-8) mol/L to 1.4×10~(-5) mol/L with a limit of detection of 3.1×10~(-8) mol/L and a sensitivity of 512 μA·mmol·L~(-1)·cm~(-2).In addition,the resulted biosensor exhibited high sensitivity,good reproducibility and long-term stability.
【Fund】: 重庆市自然科学基金资助项目(CSTC 2004BB4149);; 西南大学博士基金资助项目(SWUB2004021).
【CateGory Index】: TP212
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