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《Journal of Southwest China Normal University(Natural Science Edition)》 2008-04
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Determination of Rutin and Quercetin in Rosa Laevigata by Reversed-phase High Performance Liquid Chromatography

LIU Yan~1 GAO Zhi-xi~1 ZHOU Guang-ming~2 1.Department of Chemistry,Zunyi Normal College,Zunyi,Guizhou 563002,China;2.School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China  
A reversed-phase high performance liquid chromatography (RP-HPLC) method for the separa- tion and dertermination of the rutin and quercetin was developed.The separation was carried out on a Shim-pack VP-ODS 200 mm×4.6 mm column with a mobile phase of CH_3OH-H_2O(60:40).The flow rate was 0.8 mL/min and the wavelength of detection was 260 nm.The method had good linear relation- ship within the range 0.03—15μg/mL(r=0.999 0)and 0.04—20μg/mL(r=0.999 4)of rutin and querce- tin,respectively.The detection limit was 12.6 ng/mL and 15.2 ng/mL for rutin and quercetin,respec- tively.This method was applied for separating and determining quercetin and rutin in Rosa Laevigata.
【Fund】: 遵义市科技局资助项目(遵市科合社字(2008)23号)
【CateGory Index】: R284
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