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《Journal of Southwest China Normal University(Natural Science Edition)》 2008-06
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Study on Fingerprints of Rhizoma Alpiniae Officinarum by HPLC

WANG Li-feng, PENG Jing-dong, WANG NingSchool of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China  
To set up the HPLC fingerprints of rhizoma alpiniae officinarum.The HPLC separation was performed on a Eclipse XDB-C18(150 mm×4.6 mm,5(m) analytical column gradient eluted with methanol-acetonitrile-water(adjust to pH 3.0 with acetic acid) at the flow rate of 1.0 mL/min.The column temperature was controlled at 30 ℃,and the detection wavelength was at 254 nm.13 common peaks were found in the fingerprints of 12 batches rhizoma alpiniae officinarum,and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the herbs from different sources and the standard chromatographic fingerprint,which can be utilized for the identification of rhizoma alpiniae officinarum.The method is accurate and reproducible,providing a new reference for quality control of rhizoma alpiniae officinarum.
【CateGory Index】: R284
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