Forensic Applicability of King Fisher Method to Extract Fingerprint DNA
SUN Shuai;ZHANG Qingxia;HU Yulong;XUE Luyan;LIU Jinjie;TANG Hui;LIU Li;Jinci College of Shanxi Medical University;Beijing Judicial Identification Center of Public Security;Counter-narcotics Detachment of Ordos Public Security Bureau;
Objective To analyze the forensic applicability of King Fisher method to extract fingerprint DNA. Methods The extracting efficiency was investigated in the aspects of different swabs, deposited time of transferred fingerprint DNA, various extracting methods and diverse samples from actual cases. Results The amount of fingerprint sample, whose DNA STR loci exposure was set to no lower than 90%, was respective of 8 for flocked swab, 10 for Swab No.1 and 5 for cotton swab. With fingerprint DNA transferred by King Fisher's flocked swabs that were kept at room temperature for 1, 3, 5 and 7 days, the relevant quantity of fingerprint sample was respective of 10, 7, 6 and 4 to have their STR loci exposed no less than 90%. For the five methods of Automate, Chelex-100, Qiagen, Maxwell and King Fisher to extract fingerprint DNA, their respective performance was of 6, 4, 9, 1 and 7 samples to make their STR loci exposed ≥ 90%. Conclusion When the STR loci exposure is ≥ 60%, the maximal quantity of tested sample is reached by flocked swab(King Fisher's) to transfer fingerprint DNA although such a number is declining along with the time elapsing for the sake of DNA degradation. Therefore, it is advisable to extract DNA within 3 days. However, if the STR loci exposure is set ≥ 90%, King Fisher's is inferior to Qiagen's for their DNA extraction. With the mass of DNA template ≥ 0.01 ng to be extracted with King Fisher method, the quantity of template increases with the rfu value raising under the STR loci exposure ≥ 60% for the case samples.
【CateGory Index】： D919.2