Identification of differentially expressed genes in human uterine leiomyomas using differential display
BIN LI, MEI SUN, BIN HE, JIN YU, You DUAN ZHANG, YONG LIAN ZHANG, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, 820, Yue- Yang Road, Shanghai,
In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS:  Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named Mi in leiomyoma) were confirmed by Northern analysis.  Sequence comparison and Northern analysis proved that Li is exactly the human ribosomal protein Si9.  It was present ubiquitously in i3 tissues tested but in various levels and even in different size.  Li was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined.  No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPSi9 overexpression might be a universal signal in rapid cell growth tissues.
【CateGory Index】： Q987