Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《细胞研究(英文版)》 2002-01
Add to Favorite Get Latest Update

The molecular characterization of maize B chromosome specific AFLPs

ZHONG XIA QI, Hui ZENG, Xiu LAN LI, CHENG BIN CHEN, WEN QIN SONG, RUI YANG CHEN The College of Life Sciences, Nankai University, Tianjin 300071, China  
The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGGGTTT conserved in plant chromosome telomeres. In addition, the sequence of M8-2D shared low homology to clones from maize chromosome 4 centromere as well. M8-2D were localized to B chromosome centrorneric and telomeric regions.
【Fund】: This research was supported by grant from National Natural Sciences Foundationof China (39970357).
【CateGory Index】: Q75
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved