A STUDY ON THE RHEOLOGICAL PROPERTIES OF THE NON-METASTATIC, LUNG LOW-METASTATIC AND LUNG MID-METASTATIC CELL LINES OF THE MOUSE LUNG ADENOCARCINOMA LA795
Yu Xiaobo Institute of Basic Medical Scienccs.Chinese Academy of Medical Sciences,Beijing,100730,China
The cells of mouse lung adenocarcinoma LA 795 were divided into 15 monocolony lines. That the cell electrophoresis time (EPT) of the 15 lines were measured, among which five have significant dif- ference on the EPT) were selected. The five selected cell lines were respectively transplanted into T739 -strain inbred mice. These mice were then killied and their lung metastasis rates were examined on the 20th day after the transplantation of tumor. Through observing the lung metastasis rates, the non -metastatic (LA-1), low-metastatic (LA-D) and mid-metastatic (LA-5) tumor cell lines were obtained among the five. The following indexes, cell elcctrophoresis time (EPT), membrane fluidity, adhesion and endurance to shear stress were measured for LA-1, LA - D and LA -5. The results in -dicated 1) The cells of metastatic lines (LA - D and LA-5) have a shorter electrophoresis time and higher adhesion compared with non -metastatic line (LA-1). 2) The membrane fluidity of the three cell lines is negatively correlated with their lung metastatic rates. 3) The cell survival rate of the mid -metastatic line LA-5 was lower than that of the non-metastatic line LA-D and low -metastatic line LA- 5 on fixed action time of shear stress. These results show that there is a close relationship be- tween rheological properties of tumor cells and their invasion and metastasis. The results also imply that the increase of the blood rheological properties will raise the shear stress of blood flow , which is beneficial to kill tumor cells, and therefore may help to inhibit the metastasis of tumor.