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《Journal of Medical Science Yanbian University》 2002-04
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Determination of soyasaponin Ba and Bb in Soybean Hypocotyl by high performance liquid chromatography

QUAN Ji-shu1, YIN Xue-zhe2, KUDOU Shigemitsu3 (1.Department of Biochemistry, Yanbian University College of Medicine, Yanji 133000, Jilin, China;2.Department of Respiratory Medicine, Affiliated Hospital of Yanbian University College of Medicine, Yanji 133000, Jilin, China;3.Institute of Food Factors Science for Health, Aomori 0300842, Aomori, Japan)  
OBJECTIVETo establish a method for determination of soyasaponin Ba and Bb.METHODSSoyasaponin Ba and Bb were detected by a reversed-phase liquid chromatographic method. The separation was performed in ODS-AM-303 column (YMC, 4.6mm×250mm, 5μm), with acetonitrile-water (40∶60) containing 1mL/L trifluoroacetic acid as a mobile phase. The detect wavelength was set at 210nm.RESULTSThe method was proved to be linear in the range of 2 24~11 20μg and 2 35~11 80μg, and the intra-day coefficients of variation of assay for soyasaponin Ba and Bb were 3 15% and 3 98%, respectively. CONCLUSIONThe method is accurate and reproducible, suitable for determination of soyasaponin Ba and Bb.
【CateGory Index】: R284.1
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