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《Hereditas(Beijing)》 2005-01
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Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene Scanning Technique

HUANG Li-Guang~1,WANG Xi-Long~1,OU Jiang-Tao~1, GUO Chun-Hua~2, LI Xiao-Cheng~2,WANG Feng~1, ZHEN Xin-Li~1( 1. Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041,China)  
Wuzhishan pig is one of the rare and endangered breeds in China.They have the following characteristics such as :light body weight and small size,early sexually maturity,high meat quality and genetic identificaton with 6~8 pares litter size,body weight of born 0.3~0.4 kg,15~16 kg at 6 month old,35 kg at two years old,and so on.They may be used for laboratory utilization,comparative studies on human medical model,embryonic engineering,nutrition metabolism,sensitivity test on virus and bacteria,skin brut and tranfer,removing lipid,teeth and mouth cavity diseases,studies on cardiovascular model and evaluation of new medicine products.The polymorphisms of 32 microsatellites in 13 families of Wuzhishan pig in Hainan were analysed by multiplex PCR and gene scanning.Number of alleles in each family was counted,mean heterozygosity and polymorphism Information content(PIC) were calculated.The results showed that number of alleles was 13.66,mean heterozygosity was 0.559 while polymorphism Information content was 0.731.This revealed that genetic diversity is abundant in Wuzhishan pig in Hainan.These results have instructional significance for preserving breeds,selection and breeding,development and utilization of Wuzhishan pig in Hainan.
【Fund】: 国家"863"(编号:2001AA213151)资助项目~~
【CateGory Index】: S828.2
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