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《Hereditas(Beijing)》 2005-01
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Phylogenetic Relationship Between Tufted Deer(Elaphodus cephalophus) and Muntiacus Deer is Revealed by the Exon and Intron of K~+ Channel Gene

DAI Jun-Yong~1, CAO Xiang-Rong~1, SHI Lei~1, ZHANG Xi-Ran~1, XU Chun-Mao~2, HU Jun~2(1.College of Life Science, Nanjing Normal University, Nanjing 210097,China; 2.Wannan First-Aid Centre of Wild Animal, Xiuning 245400, China)  
In this study, partial fragments of potassium ion channel gene were amplified using the genomic DNA of muntjak, reevesi, crinifrons, and Elaphodus cephalophus.The PCR products were ligated to the plasmid of pMD18-T Vector by the method of direct T-A cloning. The positive clones were identified by colony PCR. The sequences of the recombinant clones were determined using M13-47/RV-M universal primers and aligned by the software CLUSTALW. The nucleotide divergences of exon were 0.90%~1.44% among three species of Muntiacus, and 0.90%~1.26% between E. cephalophus and each of Muntiacus deer. In the nucleotide of intron there is 0%~1.22% difference among these muntjac deers, and the divergence reached about 1.83% between E. cephalophus and the three species of Muntiacus. Using the software of MEGA to analyse molecular phylogeny, phylogenetic trees were constructed with neighbor-joining method and maximum parsimony method. The results showed that Muntiacus,crinifrons is most closely related to muntjak, while reevesi is their remote sister species and E. cephalophus is in the other genus.
【Fund】: 国家自然科学基金(编号:39970388);; 江苏省教委自然科学基金(02KJD180006)资助~~
【CateGory Index】: Q953
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