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《Hereditas(Beijing)》 2005-03
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Polymorphism Analysis of the CSN1S2 Gene Digested with Alw26Ⅰ in Five Goat Breeds

LAN Xian-Yong~(1,*), CHEN Hong~(1,2,*), ZHANG Run-Feng~1, TIAN Yi~1, LEI Chu-Zhao~1, PAN Chuan-Ying~1, LUO Jun~1,HU Shen-Rong~1(1.College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100,China; 2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China)  
PCR-RFLP was applied to analyze the polymorphism of CSN1S2 gene in 170 goats that comprised of five goat breeds, namely Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat. A 310 bp -long PCR product was digested with Alw26Ⅰ and demonstrated polymorphism in five goat populations that were all at Hardy-Weinberg equilibrium (P0.05). For Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat, gene heterozygosity/effective allele gene number/Shaanon information entropy/polymorphism information content were 0.1589/1.1889/0.2955/0.1463, 0.4114/1.6981/0.6017/0.5171, 0.1653/1.1980/0.3046/0.1516, 0.0646/1.0691/0.1463/ 0.0625, 0.0541/1.0572/0.1270/ 0.0526, respectively. According to the genetic diversity indexes described above of the five goat breeds, Guanzhong dairy goat had the most abundant genetic diversity and showed high polymorphism, and Xinong Saanen dairy goat and Shaannan white goat were inferior, while Angora goat and Boer goat had the lowest genetic variability.
【Fund】: 农业部留学人员活动基金(编号:199912D);; 陕西省自然科学基金(编号:99SM06)~~
【CateGory Index】: S827
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