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《Hereditas(Beijing)》 2005-03
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Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP

HU Xiao-Fen~(1,3),XIE Bei~(1,4),YU Rui-Song~(1,2),HUANG Qi-Zhong~1,ZHANG De-Fu~(1,2),HUANG Lu-Sheng~3,LI Zhen~(1,2)(1. Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106,China;2.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106,China;3.Jiangxi Provincial Key Laboratory for Animal Biotechnology,Jiangxi Agricultural University,Nanchang 330045,China;4.School of Life Science, Nanjing Normal University, Nanjing 210097,China)  
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
【Fund】: 上海市农业科学院中青年学术技术带头人培养基金资助~~
【CateGory Index】: S814.8
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