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《Hereditas(Beijing)》 2005-03
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Isolation and Sequence Analysis of the Xa21 Gene Exon Ⅱ Homologs from Different Species of Wild Rice in Yunnan

QIAN Jun~1,CHENG Zai-Quan~1,YANG Ming-Zhi~2, LIU Ji-Mei~1, WU Cheng-Jun~1,HUANG Xing-Qi~1(1. Key Laboratory of Biotechnology, Yunnan Academy of Agricultural Science, Kunming 650223,China; 2. College of Life Science,Yunnan University, Kunming 650091,China)  
The Xa21 gene previously cloned from the wild rice species Oryzae longistaminata confers broad-spectrum resistance to rice leaf blight caused by different strains of Xanthomonas oryzae pv. oryzae. Here we attempted to determine the existence of Xa21 homologs in other wild rice species and rice cultivars and the sequence differences between the homologs. We synthesized specific primers based on the reported Xa21 sequence to amplify homologs of the gene exon Ⅱ from several rice cultivars and three wild rice species in Yunnan Province, China. The fragments cloned from various types of O.rufipogon Griff from Jinghong and Yuanjiang, Yunnan Province, were highly homologous to the reported Xa21 gene exon Ⅱ. However, the fragment was not found in O.officinalis Wall and O.meyeriana Baill Sequence analysis suggested that differences in nucleotides were located randomly in the fragments we cloned.
【Fund】: 国家自然科学基金重点项目(编号:30069003 30460019);; 云南自然科学基金项目(编号:2004C0010Z)资助~~
【CateGory Index】: S511.9
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