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《Hereditas(Beijing)》 2005-03
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Cloning and Evolutionary Analysis of Homologous Sequences of CYP86MF Gene in Cruciferae

WANG Ling-Ping, CAO Jia-Shu, YE Wan-Zhi, XIANG Xun, ZHOU Sheng-Mao(Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, China)  
In order to direct the construction of plant germplasms by elucidating the relationship among plants at gene level, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparison indicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences in genes at nucleotide and amino acid level between species were 1.0% ~ 5.7% and 2.6% ~ 7.3% respectively, while those between genera 5.6% ~ 22.5% and 7.3% ~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus. It was concluded that CYP86MF gene was applicable to genus taxon because the differences of sequences in nucleotides and amino acids were lower (between) species than genera.
【Fund】: 国家自然科学基金(项目批准号:30370975)和浙江省重大科技项目(编号:021102536)资助~~
【CateGory Index】: Q943.2
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