Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Hereditas(Beijing)》 2005-03
Add to Favorite Get Latest Update

Cloning, Characterization and Expression Vector Construction of Potato Protease-inhibitor Ⅱ Gene(PINⅡ-2x) from Diploid Potato (Solanum phurejia)

BU Qing-Yun, WU Liang, YANG Shi-Hu, WAN Jian-Min(National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Center,Nanjing Agricultural University, Nanjing 210095,China)  
Based on the published gene sequence of tetraploid potato (Solanum.tuberosum) protease-inhibitor Ⅱ, a genomic DNA and a cDNA sequence of potato protease-inhibitor Ⅱ gene were obtained from the cDNA library and the genomic DNA of a diploid potato IVP101 (Solanum.phurejia) using PCR method and named PINⅡ-2x. Nucleotide sequencing confirmed that the full-length DNA of PINⅡ-2x was 580 bp, including an 115 bp intron and two exons. cDNA was 462 bp ( stop codon TGA not included) and had 88% similarity to the tetraploid potato protease-inhibitor Ⅱ. The PINⅡ-2x open reading frame encodes a 154-amino acid polypeptide with a predicated size of 16.6 kD and a (calculated) PI of 6.08. The deduced proteins from PINⅡ-2x cDNA had 93% homology with other tetraploid potato protease-inhibitor Ⅱ, which contain the intact signal peptide and two active site similar to the potato protease-inhibitor Ⅱ family. Test of the RT-PCR indicated that PINⅡ-2x mRNA is wound- induced expression in potato leaves. Binary vector of PINⅡ-2x cDNA drove by either rice Actin I promoter (ActⅠ) or maize Ubiquitin promoter (Ubi) was constructed.
【Fund】: 江苏省科技厅项目(编号:BG2001305)~~
【CateGory Index】: S532
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved