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《Hereditas(Beijing)》 2005-03
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Identification of Regulatory Sequence of lip Genes of Phanerochaete chrysosporium under Lignin-degradation

JIANG Ming-Feng, ZHANG Yi-Zheng(College of Life Science, Sichuan University, Chengdu 610064, China)  
Eleven subcloned DNA fragments from the 5′- upstream region of lipA, lipC and lipF of Phanerochaete chrysosporium were assayed by using the gel mobility shift assay(GMSA). The total proteins extracted from P.chrysosporium mycelia grown in Kirk medium and natural fir wood chips were used to identify the segments in these 11 DNA fragments which are bound by some regulatory proteins. The results showed that two DNA segments LG2P3(396 bp) and LG6S1-2(738 bp)in the 5′-noncoding regions of lipC and lipF were able to specifically bind total mycelial proteins of P. chrysosporium incubated in Kirk medium, respectively. One DNA segment LG6S2 (226 bp) from the 5′-noncoding region of lipF was found to specifically bind total mycelial proteins of this fungus on natural fir wood chips. Analysis of the sequences showed that there were many cis-regulatory elements in these DNA segments, implying that these sequences may be bound by some transcriptional regulation protein factors.
【Fund】: 教育部博士点基金资助项目(编号:20010610025);; 国家自然科学基金资助项目(编号:30170513)~~
【CateGory Index】: Q933
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 FENG Hong and ZHANG Yi Zheng ( Molecular Biology Laboratory, College of Life Science, Sichuan University, Chengdu 610064, China );Analysis of 5′ Upstream Regulatory Sequences of Lignin Peroxidase( LIP ) Genes of Phanerochaete chrysosporium[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1999-06
2 Jiang Mingfeng Zhang Yizheng (The College of Life Science,Sichuan University,Chengdu 610064,China);RT-PCR Analysis of Phanerochaete chrysosporium lip Genes in Colonized Fir Wood[J];Acta Microbiologica Sinica;2003-01
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 YU Yun-mei, LIU Yun, WENG En-qi, HUANG Min-sheng(Department of Environmental Science and Technology, East China Normal University, Shanghai 200062, China);Review on the production and regulation mechanism of ligninolytic enzymes of the white rot fungi[J];Journal of Safety and Environment;2005-02
2 XIE Jun, REN Lu, ZHANG Yi-zheng (Molecular Biology Laboratory,College of Life Science,Sichuan University,Chengdu, 610064,China);Production of lignocellulolytic enzymes and wheat straw degradation of Pleurotus[J];Journal of Lanzhou University;2002-02
3 LIANG Li-hua,YANG Dong-hu,KAN Zhen-rong (College of Life Science,Hebei University,Baoding 071002,China);Study on the decolorization and degradation of dye wastewater by White rot fungi[J];Journal of Biology;2003-03
4 ZHAO Chunfang 1, HU Daowei 2 (Dept. of Phor. Tech. Wuhan Ins.of Chem.Tech.,Wuhan 430073,China 2.College of Life and Sci. & Tech.,HUST,Wuhan 430073,China);Study on decolorization of several parametal complex dyes by some microbes[J];Journal of Wuhan Institute of Chemical Technology;2001-03
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 FENG Hong, ZHANG Yi zheng (The College of Life Science, Sichuan University, Chengdu 610064,China);TRANSCRIPTIONAL RESPONSIVENESS OF LIGNIN PEROXIDASE GENES TO NUTRITION IN Phanerochaete chrysosporium[J];JOURNAL OF SICHUAN UNIVERSITY (NATURAL SCIENCE EDITION);2000-S1
2 ZHANG Cheng Gang, OUYANG Shu Guang, ZHANG Shao Wen, QU Xiang Hu, YU Yong Tao, ZHOU Gang Qiao, WU Song Feng, HE Fu Chu  (Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China).;Construction and Application of a Large-scale DNA Sequence Analysis System Based on PC/Linux[J];Progress In Biochemistry and Biophysics;2001-02
3 ZHANG Cheng Gang 1) , ZHANG Li Da 2) , OUYANG Shu Guang 1) , ZHANG Qi Fa 2) , HE Fu Chu 1)** ( 1) Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China; 2) Natio;Construction and Application of the WEB Interface of Blast Package[J];Progress In Biochemistry and Biophysics;2001-06
4 FENG Hong and ZHANG Yi Zheng ( Molecular Biology Laboratory, College of Life Science, Sichuan University, Chengdu 610064, China );Analysis of 5′ Upstream Regulatory Sequences of Lignin Peroxidase( LIP ) Genes of Phanerochaete chrysosporium[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1999-06
5 Jiang Mingfeng Zhang Yizheng (The College of Life Science,Sichuan University,Chengdu 610064,China);RT-PCR Analysis of Phanerochaete chrysosporium lip Genes in Colonized Fir Wood[J];Acta Microbiologica Sinica;2003-01
6 LI Shao lan, ZHOU Bin, YANG Li yang, LI Zhi ying, ZHANG Qi, CHEN You wei (Yunnan Institute of Microbiology,Yunnan University,The Key Laboratory for Microbial Resources of Ministry of Education,Kunming 650091,China)$$$$;An improved method for extracting fungal DNA[J];Journal of Yunnan University (Natural Sciences);2002-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 FENG Hong, ZHANG Yi zheng (The College of Life Science, Sichuan University, Chengdu 610064,China);TRANSCRIPTIONAL RESPONSIVENESS OF LIGNIN PEROXIDASE GENES TO NUTRITION IN Phanerochaete chrysosporium[J];JOURNAL OF SICHUAN UNIVERSITY (NATURAL SCIENCE EDITION);2000-S1
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