Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Hereditas(Beijing)》 2005-03
Add to Favorite Get Latest Update

Three Steps for Amplifying Quick Evolving Region of DNA

QIU Gang, ZENG Qing-Tao,JIN Shan,QIAN Yuan-Huai(Faculty of Life Science, Hubei University, Wuhan 430062, China)  
A common problem in research of molecular evolution is difficult to efficiently amplify quick evolving target sequence of genes in different species or genus using specific primers, thus making experimental process and final (analysis of) total results delayed. Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D. In this research, we need PCR products corresponding to gene Fak56D of different species of Drosophila melanogaster or other genus of Drosophila. For the high evolutionary rate,most materials did not produce qualified PCR products. To solve this problem, we initially used a combination method of three steps, i.e. semi-nested PCR taken together with orientational gel extraction, which satisfactorily met the demands of next cloning and sequencing steps.
【Fund】: 国家自然科学基金重点项目(编号:39930100)~~
【CateGory Index】: Q75
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Yaping(Laboratory of cellular and Molecular Evolution,Kunming Institute of Zoology,Academia Sinica,Kunming650223,PRChina)Oliver ARyder(Center for Reproduction of Endangered Species,Zoological Society of San Diego,POBox551,San Die;Molecular Phylogeny of the Superfamily Arctoidea[J];遗传学报;1997-01
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Ming;YUAN Shi-bin;ZHANG Ze-jun;The College of Life Sciences,China West Normal University;;A Preliminary Study on Geohistoric Distributions of Giant Pandas (Ailuropoda melanoleuca)[J];西华师范大学学报(自然科学版);2013-04
2 SUN Xiao-ya1,ZHANG Zhi-he2,ZHANG Wen-ping2,SHEN Fu-jun2,WANG Yue-zhao1(1. Chengdu Institute of Biology,Chinese Academy of Science,Chengdu 610041,China; 2. The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife-The Developing State Key Laboratory for Endangered Wildlife Conservation Biology of Sichuan Province,Chengdu Research Base of Giant Panda Breeding,Chengdu 610081,China);State of Gene Flow between Captive Giant Pandas Populations[J];四川动物;2010-03
3 WANG Ya-Jun; WANG Xi-Zhong; WANG Zi-Shu; MA Yong-Xin; CHEN Wen-Yuan; WANG Xiao-Xing(Department of Biology, Sichuan University, Cheagdu 610064, China)SONG Yun-Fang; CHEN Hong-Wei; HE Guang-Xin; FEI Li-Song (Chengdu Zoological garden, Chengdu 610081, China;HETEROCHROMATIN DEMONSTRATION IN THE CHROMOSOMES OF RED PANDA (AILURUS FULGENS)[J];动物学报;1999-03
4 WANG Ya-Jun; YANG Yu-Hua; WANG Xi-Zhong(Department of Biology Sichuan University Chengdu 610064)CHEN Hong-Wei; HE Guang-Xin; FEI Li-Song; SONG Yun-Fang(Chengdu Zological Garden Chengdu 610081)YANG Yun; YU Gang(Institute of Sichuan Musk and Deer Breeding D;A Comparative Study of Banded Chromosomes of Giant Panda (Ailuropoda melanoleuca) and Black Bear (Selenarctos thibetanus)[J];遗传学报;1999-04
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Wen; Su Bing; Lan Hong; Liu Ruiqing; Zhu Chunling Nie Wenhui; Chen Yuze; Zhang Yaping (Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology,the Chiffese Academy of Set6nces, Kunming 650223);INTERSPECIFIC DIFFERENTIATION OF THE SLOW LORISES (GENUS Nycticebus) INFERRED FROM RIBOSOMAL DNA RESTRICTION MAPS[J];动物学研究;1996-01
2 Zhang Yaping Shi Liming (Laborotory of Cellular and Molecular Evolution, Kunming Institute of Zoology. Academic Sinica 650107);PREPARATION AND RESTRICTION-ENZYME ANALYSISOF MITOCHONDRIAL DNA FROM BRAINTISSUE OF MACAQUES[J];动物学研究;1992-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved