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《Hereditas(Beijing)》 2005-03
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Establishment of Immortal Lymphoblastoid Cell Lines of Non-syndromic Deafness Family and Several Methods for Transforming Human B Lyphocyte by EB virus

ZHAO Su-Ying, ZHANG Hai-Jun, XU Chun-Hong, SHAN Yun-Feng, SHAN Xiang-Nian, SHI Ting-Yan(Genetic Research Center of South-East University Medical College, Nanjing 210009, China)  
Reservation of rare family materials is the base for us to do further research. Family of Jiangsu Huaiyin is one of the biggest non-syndromic deafness families in the world. In this family,deafness is maternally inherited and all the sufferers have the mitochondrial DNA 12S RNA A1555G mutation. Four methods are used in the experiments for establishing immortal lymphoblastoid cell lines of the family with non-syndromic deafness. Results were as follows: 1 cell line was from small amout of whole blood method, 1 cell line from frozen whole blood method, 14 cell lines from frozen leukocyte method, and 36 cell lines from cyclosporin A method. In this paper, we will discuss these four methods through our experiments of establishing cell lines.
【Fund】: 国家自然科学基金项目(编号:6523001260)资助~~
【CateGory Index】: R764
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【References】
Chinese Journal Full-text Database 6 Hits
1 LI Min1, WANG Li-Bin 1,2, WANG Yu-Jiong1, MA Ai-Ying1, L Shu-Tao1 1Key Labortory of Biotechnology, Ningxia University, 2Central Laboratory, Affiliated Hospital, Ningxia Medical College, Yinchuan 750021, China;Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations[J];Journal of the Fourth Military Medical University;2007-02
2 XU Zhihua LIU Haixia Department of Otolaryngology,Shanxi Province Children's Hospital,Taiyuan 030013,China;Study on the Relationship between COCH Gene and miR-96[J];China Medicine and Pharmacy;2011-10
3 ZHANG Juan,XIONG Meng-zhen,YIN Li-hong,et,al.Key Laboratory of Environmental Medicine and Engineering,Ministry of Education;School of Public Health,Southeast University,Nanjing,Jiangsu 210009,China;Establishment of Immortalized Human Lymphocytes Cell Lines with Multiple SNP Genotyping in Chinese Han Population[J];Journal of Environment and Health;2011-01
4 CHEN Dong,YIN Wei,SHI Li-song,et al. Key Labortary of Dral Biomedical Engineering,Ministry of Education,School of Stomatology,Wuhan University,Wuhan 430074;Establishment of Immortalized Lymphoblastoid Cell Bank of Hereditary Gingival Fibromatosis Pedigrees and Their Karyotype Analysis[J];Journal of Oral Science Research;2009-03
5 LI Min~1,WANG Li-bin~(1,2),WANG Yu-jiong~(1*),LV Shu-tao~1,WANG Gui-jie~2,PAN Yue-ying~2(1.The Key Laboratory of Biotechnology,Life Science College of Ningxia University,Yinchuan 750021;2.Affiliated Hospital of Ningxia Medical College,Yinchuan 750021,China);Primary Exploration the Conditions of the Chromosome G-Banding Technique used in Examining the Human Immortal Lymphoblastoid Cells[J];Biotechnology;2006-02
6 Yuan Xiaohua,Tuerhong Keweier,Musitapa,Zuo Hongli,Qiao Yanhun,Guo Weipeng.Blood Center of Urumqi,Xinjiang 830000,China;Establishment of Immortalized B Lymphoblast Cell Lines from the Longevous Uygur People in HeTian XinJiang[J];Journal of Medical Research;2011-09
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Yan Ming ①,Liu Ningsheng ②,Shan Xiangnian ①,Xin Guangqian ③, Pu Xingkuan ③,Wu Jingyang ②,Su Zhanhai ②,Yang Huanming ④;Molecular Aenetic Study on Non-Syndromic Matrilineal Heredity Deafness[J];JOURNAL OF NANJING NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1999-01
2 LI Dong-na 1 , OU Cai-ying 2, CHEN Lu 2 (1. Department of Biology, Hainan Medical College; 2. Hospital of Hainan Medical College, Haikou 571021, China);The Establishment of Immortalized Cell Bank of the Li Nationality[J];HEREDITAS(BEIJING);1999-06
3 HUANG Xiaoyi,LIU An,YU Yang,MA Linlin,SHI Rongqian,Lü Fuqu,JIANG Yan,SUN Wenjing,XUE Yali,FU Songbin,LI Pu(Department of Medical Genetics,Harbin Medical University,Harbin 150086,China);Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the 10 Ethnic Groups in China[J];Hereditas(Beijing);2002-06
4 .Xia Bei-li;et al.(Department,u of Medical Genetics,School of Basic Medical Sciences Shanghai, Medical Universily,Shanghai,200032);Establishment of EBV-transformed lymph-oblastoid cell lines of several human genetic diseases with cyclosporin A[J];CHINESE JOURNAL OF BIRTH HEALTH & HEREDITY;1995-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Dong na, YING Da jun, OU Cai ying;Establishment and preservation of permanent growing B cell line of Li nationality in Hainan province of China[J];Acta Academiae Medicinae Militaris Tertiae;2003-15
2 LI Min1, WANG Li-Bin 1,2, WANG Yu-Jiong1, MA Ai-Ying1, L Shu-Tao1 1Key Labortory of Biotechnology, Ningxia University, 2Central Laboratory, Affiliated Hospital, Ningxia Medical College, Yinchuan 750021, China;Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations[J];Journal of the Fourth Military Medical University;2007-02
3 WANG Li-Xin1,WEI Jun1,YANG Bao-Zhen1,PAN Yue-Ying1,ZHANG Dong2,SHI Xin-Rong2 Medical Laboratory Center,Affiliated Hospital,Ningxia Medical University,Yinchuan 750004,China;Establishment and significance of immortal lymphoblastoid cell lines of familial adenomatous polyposis[J];Journal of the Fourth Military Medical University;2009-18
4 YAN Xin, GAO Jian-hua, SONG Mei, LIU Xiao-jun, CHEN Yang Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Establishment of immortalized lymphoblastoid cell bank of a keloid pedigree[J];Journal of Southern Medical University;2006-01
5 SONG Mei,GAO Jian-hua,YAN Xin,LIU Xiao-jun,CHEN Yang Department of Plastic Surgery,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Establishment of immortalized B-lymphoblastoid cell lines of keloid pedigree and its karyotype analysis[J];Journal of Southern Medical University;2006-12
6 ZHAO Su-ying, LI Min, LI Ke-juan(Department of Laboratory Medicine, Jiangsu Provincial Hospital of TCM, Nanjing, Jiangsu, 210029);Comparison of Several Methods of Transformin Human B Lyphocyte by EB virus[J];Hainan Medical Journal;2007-12
7 Zeng Liping Gao Feng Mei Guisen Liu Ping Gao Jinlai Liu Fulin Department of Clinical Laboratory,The 261st Hospital of PLA (Beijing 100094);ESTABLISHMENT OF PERMANENT LYMPHOBLASTOID CELL BANK OF SCHIZOPHRENIC PATIENTS IN CHINA[J];Journal of Hebei Medical University;2001-03
8 LIU Yu,ZHAO Yu-hua(Harbin Institute of Physical Education,Harbin 150008,HeilongJiang China);The Feasibility Analysis of Cell Line Immortalization in Athletics[J];Journal of Harbin Institute of Physical Education;2011-01
9 WANG Ping-zhong, BAI Xue-fan, PAN Lei, HUANG Chang-xing, LING Jin-ge, HAO Chun-qiu (The Center of Diagnosis and Treatment of Infectious Diseases of PLA, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, Shaanxi, China);Establishment and its significance of B-lymphoblastoid cell lines in patients with hemorrhagic fever with renal syndrome[J];Journal of Medical Postgraduates;2003-11
10 CHEN Dong,YIN Wei,SHI Li-song,et al. Key Labortary of Dral Biomedical Engineering,Ministry of Education,School of Stomatology,Wuhan University,Wuhan 430074;Establishment of Immortalized Lymphoblastoid Cell Bank of Hereditary Gingival Fibromatosis Pedigrees and Their Karyotype Analysis[J];Journal of Oral Science Research;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Zhen,ZOU Hongyan,WU Guoguang,et al.Shenzhen Institute of Transfusion Medicine, Shenzhen 518035 ,China;Prepae B-lymphoblastoid cell lines of HLA novel allele B ~*5610 in a family[J];Chinese Journal of Blood Transfusion;2004-05
2 LI Min1, WANG Li-Bin 1,2, WANG Yu-Jiong1, MA Ai-Ying1, L Shu-Tao1 1Key Labortory of Biotechnology, Ningxia University, 2Central Laboratory, Affiliated Hospital, Ningxia Medical College, Yinchuan 750021, China;Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations[J];Journal of the Fourth Military Medical University;2007-02
3 WANG Li-Xin1,WEI Jun1,YANG Bao-Zhen1,PAN Yue-Ying1,ZHANG Dong2,SHI Xin-Rong2 Medical Laboratory Center,Affiliated Hospital,Ningxia Medical University,Yinchuan 750004,China;Establishment and significance of immortal lymphoblastoid cell lines of familial adenomatous polyposis[J];Journal of the Fourth Military Medical University;2009-18
4 Zheng Chenguang,Long Guifang Yang Ze,et al.(Guangxi Jiangbin Hospital,Nanning 530021 China);ESTABLISHING THE CELL-STORAGE OF IMMORTAL CELL LINES FROM THE LONGVOUS RESIDENTS OF ZHUANG NATIONALITY IN LONGEVITY VILLAGES IN BAMA, GUANGXI[J];Journal of Guangxi Medical University;2003-01
5 Zeng Liping Gao Feng Mei Guisen Liu Ping Gao Jinlai Liu Fulin Department of Clinical Laboratory,The 261st Hospital of PLA (Beijing 100094);ESTABLISHMENT OF PERMANENT LYMPHOBLASTOID CELL BANK OF SCHIZOPHRENIC PATIENTS IN CHINA[J];Journal of Hebei Medical University;2001-03
6 LI Dong-na,YING Da-jun,OU Cai-ying,et al. (Department of Biology,Hainan Medical College,Haikou571101,China);ESTABLISHMENT AND PRESERVATION OF IMMORTAL LYMP-HOBLASTOID CELL LINESOF THE HA-LI,THE QI-LI AND THE BENDI-LI ETHNIC GROUPSIN HAINAN ISLAND,CHINA[J];Journal of Hainan Medical College;2004-01
7 JIN Ying 1 TANG Mei 2 LI Guoming 3 (1 Laser and Life Science Institute, 2 Biotechnology Institute, 3 Chemistry Department, South China Normal University, Guangzhou, 510631);A Practical Culture Technique in Lymphocytes[J];ACTA LASER BIOLOGY SINICA;2000-01
8 PAN Xiu-zhen,YUE Jian-hua,LI Xian-fu,WANG Chang-jun,TANG Jia-qi(Huadong Research Institute for Medicine and Biotechniques,Nanjing 210002,Jiangsu,China);Establishment of Epstein-Barr virus-transformed B cell lines from healthy volunteers of type B[J];Journal of Medical Postgraduates;2008-01
9 YAN Han-song, HUANG Chang-bo, YE Xiao-qian , et al. Department of Oral Maxillofacial Surgery, Hospital of Stomatology, Xiangfan City,,Xiangfan 441003;Clinical and Genetic Features of Chinese Kindreds with Autosomal Dominant Hereditary Gingival Fibromatosis.[J];Journal of Comprehensive Stomatology;2005-03
10 CHEN Dong,YIN Wei,SHI Li-song,et al. Key Labortary of Dral Biomedical Engineering,Ministry of Education,School of Stomatology,Wuhan University,Wuhan 430074;Establishment of Immortalized Lymphoblastoid Cell Bank of Hereditary Gingival Fibromatosis Pedigrees and Their Karyotype Analysis[J];Journal of Oral Science Research;2009-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Li-Xin1,WEI Jun1,YANG Bao-Zhen1,PAN Yue-Ying1,ZHANG Dong2,SHI Xin-Rong2 Medical Laboratory Center,Affiliated Hospital,Ningxia Medical University,Yinchuan 750004,China;Establishment and significance of immortal lymphoblastoid cell lines of familial adenomatous polyposis[J];Journal of the Fourth Military Medical University;2009-18
2 ZHANG Juan,XIONG Meng-zhen,YIN Li-hong,et,al.Key Laboratory of Environmental Medicine and Engineering,Ministry of Education;School of Public Health,Southeast University,Nanjing,Jiangsu 210009,China;Establishment of Immortalized Human Lymphocytes Cell Lines with Multiple SNP Genotyping in Chinese Han Population[J];Journal of Environment and Health;2011-01
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 YU Min, ZHANG Yong Li, CHEN Feng, XUE Ya Li, YU Yang, MA Lin Lin, HUANG Xiao Yi, LIU An, SHI Rong Qian, L Fu Qu, HUANG Cheng Bin, ZHANG Gui Yin, LI Pu, FU Song Bin ① (Biology Department and Medical Genetics Laboratory of Harbin Medical Univ;Y Chromosome Variations and Hyplogroups from 15 Biallelic Markers in Six Chinese Populations[J];Acta Genetica Sinica;2002-04
2 LI Dong-na 1 , OU Cai-ying 2, CHEN Lu 2 (1. Department of Biology, Hainan Medical College; 2. Hospital of Hainan Medical College, Haikou 571021, China);The Establishment of Immortalized Cell Bank of the Li Nationality[J];HEREDITAS(BEIJING);1999-06
3 XUE Ya li,WANG Qi,SHI Zhong cheng,LIU An,ZHANG Yu,HUANG Xiao yi,HUANG Cheng bin,CHEN Bai bin,YANG Huan jie,FU Song bin,LI Pu(Laboratory of Medical Genetics,Department of Medical Biology,Harbin Medical University,Harbin 150086,China);Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China[J];HEREDITAS(BEIJING);2000-03
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