Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Hereditas(Beijing)》 2005-03
Add to Favorite Get Latest Update

Construction, Expression, Purification and Antiviral Activity of Fused Interferon-BLA (IFN-BLA)

MA Wen-Zhe, SHEN Qiong, HAN Wei, LU Chen-Yi, YANG Sheng-Li, GONG Yi (Shanghai CASB Biotechnology CO., LTD, Chinese Academy of Sciences, Shanghai 200233, China)  
A fusion protein, Interferon-BLA (IFN-BLA), was constructed with IFN-beta-1b and IFN-alpha-2b separating by a linker -GGGS-. The laboratory-scale expression conditions in E.coli BL21 CodonPlus (DE3)-RIL were optimized and IFN-BLA was expressed higher than 35% of total protein in the cells mainly as inclusion body. The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography. The overall yield of IFN-BLA was about 45 mg/L with purity higher than 90%. Antiviral activity assay suggested that this newly fused (protein) may have synergetic or additive antiviral activities.
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Liu Changnuan, Liu Lan, Wang Junzhi et al(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050);A Colorimetric Method for Determination of IFN Potency[J];CHINESE JOURNAL OF BIOLOGICALS;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIN Ting,GUAN Yixin,FEI Zhengzheng and YAO Shanjing (Department of Chemical and Biochemical Engineering,Zhejiang University, Hangzhou 310027,Zhejiang,China);RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION[J];Journal of Chemical Industry and Engineering(China);2004-05
2 CHEN Zhi-yong,LIU Bo,DENG Jie(Chengdu Insitute of Biological Proclucts,Chengdu 610023,China);Fitting dose-effect curve with the accumulation normal distribution[J];West China Journal of Pharmaceutical Sciences;2007-01
3 GUAN Yi-Xin **, FEI Zheng-Zheng, LUO Man, YAO Shan-Jing (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China);Minichaperone (GroEL191~345)-mediated In vitro Refolding of Recombinant Human Interferon-γ Inclusion Body[J];Progress In Biochemistry and Biophysics;2004-10
4 REN Yu-Ying1,2,FAN Quan-Shui2,QIU Wei2,LI Zuo-Sheng2,ZHENG Ying2,ZHANG Fu-Qiang2,DU Qiang1,2,YU Fang-Fang1,2 1.College of Animal Science and Technology,Yunnan Agriculture University,Kunming 650201;2.Military Medical Institute of Chengdu Military Area Center of Disease Prevention Control,Kunming 650032;China;Preparation and Purification of Recombinant Canine Interferon Alpha[J];Letters in Biotechnology;2009-01
5 Liu Changnuan,Liu Lan,,Zhang Yi et al( National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050 );Quality Control Methods for Chemical Modified Interferon[J];Chinese Journal of Biologicals;2000-04
6 DAI Hua,JI Yan,ZHENG Jia-Yu,XU Zheng-Zhong,PAN Zhi-Ming,JIAO Xin-An Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou 225009,China;Expression of Recombinant Chicken IFN-γ in Baculovirus Vector System[J];Letters in Biotechnology;2009-06
7 FEI Zheng-Zheng GUAN Yi-Xin YAO Shan-Jing ( Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027);A Colorimetric Method to Assay Biological Activity of Recombinant Human IFN-γ[J];Microbiology;2004-03
8 KONG Gui-mei XU Jin-jun QIN Ai-jian JIN Wen-jie LIU Yue-long(Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University,Yangzhou\ 225009, China);High level expression of recombinant chicken interferon-gamma in insect cells[J];Acta Microbiologica Sinica;2005-05
9 YANG Li WANG Dong-Kai LIU Qian BI Dian-zhou College of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China LIN Hua-qing Guangdong College of Pharmacy,Guangdong 510224,China;Study on the method for determination of entrapment efficiency of interferon α-2b liposomes[J];Chinese Journal of Pharmaceutical Analysis;2007-03
10 JIN Ting~(1,2),GUAN Yi-xin~1,LIN Dong-qiang~1,YAO Shan-jing~(1)(1.Department of Chemical and Biochemical Engineering,Zhejiang University,Hangzhou 310027,China; 2.College of Biochemical Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100,China);Refolding of recombinant human interferon-gamma by expanded bed adsorption chromatography with urea gradient[J];Journal of Zhejiang University(Engineering Science);2006-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved