Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Hereditas(Beijing)》 2005-03
Add to Favorite Get Latest Update

A Novel Method for Increasing the Number of Metaphase Cells Cultured in vitro

HOU Ying~(1, 2), TAN Li-Xin~1, LI Wen-Rong~1, NIU Zhi-Gang~1,GUO Zhi-Qin~1(1. Key Laboratory of Livestock Reproduction and Breeding Biotechnology of Ministry of Agriculture,Xinjiang Academy of Animal Science,Urumqi 830000, China;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046, China)  
To increase the number of metaphase cells cultured in vitro, two bovine fibroblast cell lines(YFF and CNF) were frozen at 4℃ for different length of time prior to colchicine treatment, and then chromosomal specimen were prepared. The percentage of metaphase cells was examined under conditions above. Using this method, the variation rate of karyotype of YFF and CNF subcultured up to passage 20 were also analyzed. It was found that the percentage of YFF and CNF metaphase cells in treatment group were significantly higher than that in control group(P0.05), and the number of YFF and CNF metaphase cells obtained in 20 h treatment group were increased more than 5 fold as many as that in control group(P0.01), 31.7%and 40.2% vs 4.7%and 6.4%, respectively. These data suggest that the method of freezing at 4℃ could be used for increasing the number of metaphase cells in vitro conveniently, and analyzing the variation rate of karyotype of cultured cells efficiently.
【Fund】: 新疆高技术研究发展计划项目。家畜乳腺生物反应器关键技术平台的建立与应用(批准号:200311105)~~
【CateGory Index】: Q813.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 DUO Shu-guang, WU Ying-ji, LUO Fen-hua, BOU Shorgan (Key Laboratory of China Education Ministry for Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, China);Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells[J];Zoological Research;2006-03
2 YIN Ming1,ZHANG Yi-zhe1,DUO Shu-guang2 (1. Beijing VitalStar Biotechnology Co. Ltd. Beijing 100107,China) (2. College of Life Sciences,Peking University,Beijing 100871,China);Isolation,Culture and Related Biological Characteristics of Bovine Fetal Fibroblasts[J];Laboratory Animal Science;2010-03
Chinese Journal Full-text Database 2 Hits
1 LIU Ji-Long;WANG Min-Kang; LI Jin-Song;SUN Qing-Yuan;XU Zhi CHEN Da-Yuan(State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Science, Bijing 100080, China)(Tianjin Husbandry & Veterinary Institute, Tianjin 300112;CULTURE OF BOVINE EAR FIBROBLASTS[J];ACTA ZOOLOGICA SINICA;1999-04
2 Wang yiping et al.:;The Methods of Preparing Early Metaphase Chromosomes and the Study on Their G-banding Patterns in Mouse Fibroblasts[J];Hereditas(Beijing);1989-01
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yun-hai,ZHANG Xiao-rong,LIU Ya,JIN Ren-tao, JU Shu-cun,ZHANG Mei-ling,WANG Cun-li (College of Animal Sciences and Fishery,Anhui Agricultural University,Hefei 230036);Culture in Vitro of Fibroblasts Cells of Boer Goat and Yellow Cattle′s Ear Skin[J];Journal of Anhui Agricultural University;2002-04
2 YANG Xiao Jing YAO Hong Jun WANG Yu Feng (College of Life Science, Central China Normal University, Wuhan 430079, China);Advances in the Research of Oocyte Apoptosis in Mammals[J];Chinese Journal of Zoology;2004-06
3 ZHANG Ming~① HOU Rong~(②) ZHENG Hong-Pei~① ZHU Qing~①ZHANG Zhi-He~② LIU Yu-Liang~②(① College of Animal Science & Technology,Sichuan Agricultural University,Ya′an 625014;② Giant Panda Breeding Research Base in Chengdu,Chengdu 610081,China);Establishment and Cryopreservation of a Giant Panda Skin Fibroblast Cell Line[J];Chinese Journal of Zoology;2005-05
4 GAO Qian-jin~1,LI Zhuang-zhi~1,MA Xin-dong~2,WANG Rui-yuan~3(1.Dept.of P.E.,Shijiazhuang University,Shijiazhuang 050035,Hebei China;2.Tsinghua University Beijing 100084,China;3.Beijing Sport University,Beijing 100084,China);Reviews on Skeletal Muscular Cytoskeletal Proteins[J];Journal of Beijing University of Physical Education;2005-10
5 WANG Hong-mei 1, HUANG Jun-cheng 2,LIU Ming-jun 2, WANG Li-hu 3,JI Qiao-ling 1,GUO Zhi-qin 2 (1.Department of Biology XinJiang University ,Urumqi 830046; 2.Key Laboratory of Animal & Veterinary Biotechnology of MOA,XinJiang Academy of animal Scien;Sdudy on in vitro culture of fibroblast cells using animal ear culture[J];Herbrvorous Animals;2001-S2
6 WANG Li-hu~1, HUANG Jun-cheng~2, WANG Hui-chuan~1, SHI Qiao-ting~3, GUO Zhi-qin~2 (1. Department of Biotechnology, Beijing Agricultural College, Beijing 102206, China; Academy of Animal Science, Urumqi 830000, China; 3. Institute of Biotechnology in Henan Academy of Agricultural Science, Zhengzhou 450002, China);Effect of in Vitro Maturation Times of Oocytes on Development of Sheep Nuclear Transfer Embryos[J];Grass-feeding livestock;2004-04
7 WANG Duan-ming~(1,2),YI Zhong~3, HAI-Liqiemu~(1,3), CHEN Jing-bo~(1,3)(1. Xinjiang TECON Animal Husbandry Biotechnology Co. Ltd,Urumqi 830000,China; 2. College of Animal Science, Xinjiang Agriculture University, Urumqi 830052,China;3. Xinjiang Academy of Animal Science, Urumqi 830000 China);The Skin Fibroblasts in Vitro Culture in Bovine[J];Grass-feeding livestock;2005-02
8 MEN Zhong-hua,LI Sheng-xiu(College of Natural Resources and Environment Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Progress in isolation of vacuoles of higher plants[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
9 ZHAO Bo, RAO Jing-ping (College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Changes of Cell-wall Polysaccharides and Their Catabolic Enzyme Activities of Persimmon Fruits during Post-harvest[J];Acta Botanica Boreali-occidentalia Sinica;2005-06
10 DING Sheng-cai1, CHEN-Yi-sheng1, WEI Hong2, LIU Ping3 (1Research Institute of Pathology, Southwest Hospital, 2Department of Laboratory Animal Science, 3Department of Obstetrics and Gynecology, Xinqiao Hospital, 400037, Third Military Medical University, Chongqing 400038, China);Cultivation and identification of porcine skin fibroblasts[J];Acta Academiae Medicinae Militaris Tertiae;2004-13
Chinese Journal Full-text Database 8 Hits
1 Shan Xiang-nian, Chen Yi-feng, Luo Li-hua, Cao Xiao-mei, Song Ji-zhi (Kunming Institute of Zoology, Academia Sinica) Zeng Yang-zhi (Yunnan University of Agriculture);Comparative Studies on the Chromosomes of Five Species of Catties of the Genus Bos in China[J];Zoological Research;1980-01
2 Zhou Qi (Institute of Zoology, CAS, 100080 Beijing);Generation of Fertile Cloned Rats by Regulating Oocyte Activation[J];Bulletin of the Chinese Academy of Sciences;2003-06
3 WT5BZ]Li Zhen Wang Ying Liu Huili Zhang Defu [WT5BX](Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Laboratory of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agro genetics a;STUDY ON ISOLATION AND CULTURE OF BOVINE [WT4HZ]MAMMARY EPITHELIAL CELLS[J];ACTA AGRICUL TURAE SHANGHAI;2000-03
4 Xu Manni 1,2 Li Shuguang 1 Zhao Jianyang 2 Cheng Guoxiang 2 ( 1 Dept of Preventive Medicine College of Medicine Tong Ji University, Shanghai 200331; 2 Transgenic Research Center of Shanghai, Shanghai 201203);The Detection Method of Mammary Gland Bioreactor Expression Vector[J];Biotechnology Information;2003-05
5 OUYANG Wu qing,QIAN Ju fen (College of Animal Sciences and Technology,Northwestern Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Establishment of culture system in goat mammary epithelial cell[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2003-03
6 ZHENG Yue-mao,PENG Xin-rong,XU Yong-ping,SHI Yu-qiang,ZHANG Yong(Institute of Bio-Engineering,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Study on appearance of goat mammary epithelialcells which cultured in vitro[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2004-03
7 LI Yang GUO Ji Tong WU Kai Feng BAO Shorgan ( The Research Center for Laboratory Animal Science of Inner Mongolia University The Key Laborayoty of Ministry of Education of China for Mammal Reproduction Biology and Biotechnolgoy, Huhhol 010021);DISSOCIATION AND IN VITRO CULTURE OF BOVINE FIBROBLASTS[J];Chinese Journal of Coll Biology;2002-04
8 Wang yiping et al.:;The Methods of Preparing Early Metaphase Chromosomes and the Study on Their G-banding Patterns in Mouse Fibroblasts[J];Hereditas(Beijing);1989-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 FU Guo-wen et al(College of Animal Science and Technology,Northwest A & F University of Sciences and Technology University,Yangling,Shaanxi 712100);Goat Mammary Epithelial Cells Cultured by Digestion Method[J];Journal of Anhui Agricultural Sciences;2007-24
2 ZHUANG Xin-jie et al(Animal Reproduction Institute,Guangxi University,Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization,Nanning,Guangxi 530005);Isolation,Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo(Bubalus bubalis) Endometrium[J];Journal of Anhui Agricultural Sciences;2009-18
3 BI Lin et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Research Progress of Primary Culture Method of Mice Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-07
4 TIAN Tian et al (College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Optimization of Parameters of Exogenous Gene Transfection of Yak Mammary Epithelial Cells in vitro Mediated by Liposome[J];Journal of Anhui Agricultural Sciences;2010-09
5 CHEN Jianhui,GAO Xuejun,LI Qingzhang (Key Laboratory of Dairy Sciences,Ministry of Education,College of Food,Northeast Agricultural University,Harbin 150030,China);Research progress of culture technique on diary cow mammary gland epithelial cells in vitro[J];Journal of Northeast Agricultural University;2009-07
6 YAN Jing,HE Xiao-ying,PAN Chun-liu,LAN Zhi-gang,ZHANG Yong (College of Veterinary Medicine,Institute of Bio-engineering,Northwest A & F University,Yangling,Shaanxi,712100,China);Construction of Eukaryotic Expression Vector pEGFP-hTERT and the Optimization of Scheme on Bovine Mammary Cell Transfection[J];Progress in Veterinary Medicine;2009-05
7 GONG Chao,WANG Ling-yun,ZHOU Huan-min(College Of Life Sciences,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia,010018,China);Study on Culture of Bovine Mammary Epithelial Cells in Vitro[J];Progress in Veterinary Medicine;2011-07
8 LI Xi-yan,WANG Jia-qi,WEI Hong-yang,BU Deng-pan,HU Han,ZHOU Ling-yun(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Bovine Mammary Epithelial Cells Culture Methods in vitro and Its Application[J];Acta Agriculturae Boreali-Sinica;2010-S2
9 LI Zhong-qiu(Animal Husbandry Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086);Progress of the Bovine Mammary Epithelial Cells[J];Heilongjiang Agricultural Sciences;2008-05
10 ZHUANG Xin-jie, DUAN Ya-ping, HUANG Yi, NING Shu-fang, ZHANG Ming Animal Reproduction Institute, Guangxi University, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530005;Isolation, Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo (Bubalus bubalis) Endometrium[J];农业科学与技术(英文版);2009-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved