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《Hereditas(Beijing)》 2005-03
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A Novel Method for Increasing the Number of Metaphase Cells Cultured in vitro

HOU Ying~(1, 2), TAN Li-Xin~1, LI Wen-Rong~1, NIU Zhi-Gang~1,GUO Zhi-Qin~1(1. Key Laboratory of Livestock Reproduction and Breeding Biotechnology of Ministry of Agriculture,Xinjiang Academy of Animal Science,Urumqi 830000, China;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046, China)  
To increase the number of metaphase cells cultured in vitro, two bovine fibroblast cell lines(YFF and CNF) were frozen at 4℃ for different length of time prior to colchicine treatment, and then chromosomal specimen were prepared. The percentage of metaphase cells was examined under conditions above. Using this method, the variation rate of karyotype of YFF and CNF subcultured up to passage 20 were also analyzed. It was found that the percentage of YFF and CNF metaphase cells in treatment group were significantly higher than that in control group(P0.05), and the number of YFF and CNF metaphase cells obtained in 20 h treatment group were increased more than 5 fold as many as that in control group(P0.01), 31.7%and 40.2% vs 4.7%and 6.4%, respectively. These data suggest that the method of freezing at 4℃ could be used for increasing the number of metaphase cells in vitro conveniently, and analyzing the variation rate of karyotype of cultured cells efficiently.
【Fund】: 新疆高技术研究发展计划项目。家畜乳腺生物反应器关键技术平台的建立与应用(批准号:200311105)~~
【CateGory Index】: Q813.1
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