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《Hereditas(Beijing)》 2005-06
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Isolation and Sequence Analysis of Aω-Gliadin Homologous Gene from Wheat

CHEN Fan-Guo,XIA Guang-Min(School of Life Science,Shandong University,Jinan 250100,China)  
The DNA sequence of a full-length Triticum astivum CV.Jinan 177ω-gliadin homologous gene(ω1236) containing partial 5′and 3′ flanking sequences with no intron was cloned by genomic PCR-based technology.The ω1236 sequence possibly encoded a putative 47.2 kDa protein except for eight stop codons at amino acid residue positions 87,117,125,157,198,313,357 and 365 respectively.All the eight stop codons were caused by base transition.Sequence analysis revealed that ω1236 had 98% homology to a ω-gliadin gene of wheat(AB059812).Like all other gliadin gene families characterized in cereals,this gene possessed all the features in other plant reported previously.Phylogenetic analysis of the completely sequenced gene as well as those ω-gliadin genes in wheat,ω-secalin and C-horden genes in rye and barley,and α-,β-andγ-gliadin genes in wheat indicated that the ω1236 was more closely related to ω-gliadin gene family,much less homology to α-,β-and γ-gliadin gene families.Short peptide was produced in the culture of transformed E.coli induced by IPTG in early 2 h.It indicated that stop codon would be in ω1236.The result is consistent with that of the sequenced gene.The present paper could accumulate data useful for both ω-gliadin gene cloning by PCR and the study on structures and functions of these genes.
【Fund】: 国家自然科学基金(编号:30370857)资助~~
【CateGory Index】: S512.1;
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【Co-citations】
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1 LIU Chang et al(School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu,Sichuan 610054);Cloning and Sequence Analysis of α-gliadin Gene from Triticum timopheevii[J];Journal of Anhui Agricultural Sciences;2006-18
2 LI Guang-rong et al(School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu,Sichuan 610054);Cloning and Sequence Analysis of An α-gliadin Gene from Australopyrum retrofractum[J];Journal of Anhui Agricultural Sciences;2007-27
3 HU Yun et al (Institute of Wheat Culture,Guizhou University,Guiyang,Guizhou 550025);Study on the Subunit Composition of High Molecular Weight Glutenin in Wild Emmer[J];Journal of Anhui Agricultural Sciences;2008-01
4 WANG Xin~1,FAN San-hong~(1,2*),YAN Xiao-hua~1,LUO Long-hai~1,GUO Ai-guang~(1,2)(1 College of Life Sciences,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Key Laboratory of Agriculture Molecule Biology,Yangling,Shaanxi 712100,China);Developing Specific Molecular Marker for Wheat HMW-GS1Bx14 Gene[J];Acta Botanica Boreali-occidentalia Sinica;2005-11
5 PENG Yan,ZHANG Jin-rui,LIU Yong,HE Guang-yuan (China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,College of Life Science and Technology ,Huazhong University of Science and Technology, Wuhan 430074,China);Composition Analysis of High-Molecular-Weight Glutenin Subunits of Semi-natural Wheat (Triticum aestivum ssp.tibetanum Shao) in Tibet[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
6 WU Fang,PAN Zhi-fen2,YU Mao-qun (Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);Research Advances about Low Molecular Weight Glutenin Subunits in Wheat[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
7 CHEN Ming-jie~1,FANG Ti~2,LIU Yong~1,HUANG Wei-wei~1,YANG Guang-xiao~1,HE Guang-yuan~ 1* (1 China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,China;2 Biotechnology Base,College of Life Science,Wuhan University,Wuhan 430072,China);Cloning and Expression of Pinb Gene in Aegilops bicornis[J];Acta Botanica Boreali-Occidentalia Sinica;2006-06
8 YAN Hua-xiao~1,ZHAO Hui~(1*),WANG Xian-ze~2,WANG Fang~2(1 Department of Biotechnology,College of Chemical and Environmental Engineering,Shandong University of Science and Technology,Qingdao,Shandong 266510,China;2 Key Laboratory of Crop Biology of Shandong Province,Shandong Agricultural University,Tai'an,Shandong 271018,China);AS-PCR Marker of HMW-GS 1Bx14 Related to Baking Quality[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
9 PANG Yu-hui1,CHEN Xin-hong1,ZHAO Ji-xin1,WU Jun1,CHENG Xue-ni2,LIU Shu-hui1,YANG Qun-hui1,DU Wan-li1,CHEN Lin-gang1(1 College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;2 College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China);Cloning and Sequence Analysis of the HMW-GS Gene and Its Promoter from Dasypyrum villosum[J];Acta Botanica Boreali-Occidentalia Sinica;2009-05
10 Chen Mingjie1,2 He Guangyuan1* Yang Guangxiao1 YaoQin1 Chang Junli1 Peter Shewry21 China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan, 430074; 2 Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK;Cloning and Expression Analysis of Puroindoline Genes in Aegilops kotschyi[J];Molecular Plant Breeding;2005-06
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