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《Hereditas》 2008-01
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Cloning and analysis of adenine nucleotide translocase gene in Helicoverpa armigera

NIU Bao-Long, WENG Hong-Biao, HE Li-Hua, SHEN Wei-Feng, QI Xiao-Peng, LIU Yan, MENG Zhi-Qi Laboratory of Entomo-molecular Biology, Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China  
A cDNA clone encoding the ADP/ATP translocase in Helicoverpa armigera has been identified by RT-PCR, 5′ and 3′RACE methods. Sequence analysis shows that it is 1 190 bp long and contains a single open reading frame (ORF, 133~1 033 bp) encoding a protein of 300 amino acids (GenBank submission number, AY253868). The protein has a 22 aa signal peptide on its N-terminal, which leads the protein locating onto the inner membrane of the mitochondria. It also has three conserved domains of the mitochondrial carrier protein forming a channel to exchange ATP and ADP energy molecule through the inner membrane of the mitochondria. It shows extensive similarities to the known ADP/ATP translocase polypeptides. The ADP/ATP translocase similarity was up to 90% in the Lepidoptera.
【Fund】: 国家科技攻关项目(编号:2004DFBA0007)资助~~
【CateGory Index】: Q78
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