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《Hereditas》 2008-01
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Cloning and analyzing of rice blast resistance gene Pi-ta~+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan

GENG Xian-Sheng1, 2, YANG Ming-Zhi1, HUANG Xing-Qi2, CHENG Zai-Quan2, FU Jian2. SUN Tao3, LI Jun3 1. School of Life Science, Yunnan University, Kunming 650091, China;2. Biotechnology and Germplasm Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;3. Agricultural Institute of Xishuangbanna Prefecture, Jinghong, Yunnan Province 666100, China  
A4672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid sequence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics. Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.
【Fund】: 云南省自然科学基金重点项目(编号:2004C0010Z);; 云南省自然科学基金(编号:2006C00007Q)~~
【CateGory Index】: S511.9
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