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《Hereditas》 2008-01
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A method of extraction and separation of wheat gluten

JI Jun1,2, LIU Dong-Cheng2, WANG Jing1, LI Jun-Ming1, ZHANG Ai-Min2 1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, CAS, Shijiazhuang 050021, China;2. National Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Acad-emy of Sciences, Beijing 100101, China  
After removing gliadin and other proteins with 7.5% 2-propanol and 0.3 mol/L NaI, the total glutenin subunits were extracted with two kinds of buffer, one containing 25% 2-propanol, 0.04 mol/L Tris-HCI (pH=8.0), 10% SDS and 2% DDT and the other containing 25% 2-propanol, 0.04 mol/L Tris-HCI (pH=8.0), 10% SDS and 1.4% VP. In SDS-PAGE electrophoresis (4% stacking gel and 13% resolving ge1) system HMW-GS and LMW-GS were clearly separated by the improved method with only a single step. The background of the SDS-PAGE gel was gliadin-free. It was more effective and applica ble to separate and distinguish the complex LMW-GS. This method will be used in wheat protein separation, variety identification and improvement.
【Fund】: 国家重点基础研究项目(编号:2002CB111301);; 高技术研究发展计划(No.2006AA10Z1B2)~~
【CateGory Index】: S512.1
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