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《Hereditas》 2008-09
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Progress on methods for purity assessment of separated chromosome X-or Y-bearing sperm

GUO Jia-Ming1,2, ZHU Hua-Bin1, WANG Dong1, ZHANG Lin-Bo2, HAO Hai-Sheng1, DU Wei-Hua1 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. College of Life Sciences Jilin Agricultural University, Changchun 130118, China  
In this review of methods for purity assessment of isolated chromosome X-and Y-bearing sperm, we compared the principles, operating procedures, as well as pros and cons for various methods. We conclude that nested PCR of single sperm will become a conventional and popular method with lower costs, and the method will play a very important role in optimizing the X, Y sorting method, if the sensitivity and accuracy of the method can be increased and the testing time de-creased, and promote the new progress in other genetic testing techniques on single sperm.
【Fund】: 国家自然科学基金资助项目(编号:30671502);; 国家高技术研究发展规划(863项目)项目(编号:2006AA10Z143)资助~~
【CateGory Index】: Q813
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