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《Hereditas》 2008-09
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Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene

LIU Qing1, WU Shun1, SHEN Ge-Zhi2, PENG Qiong1, LIU Tao-Li1, CHENG Peng1, XIAO Lang-Tao1 1. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China; 2. Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China  
The segregation of exogenous genes was studied by hygromycin-resistant and PCR experiments in the transgenic rice (Oryza sativa L. subsp. japonica and indica) with anti-sense waxy gene, meanwhile the change of amylose and waxy protein contents in progenies of transgenic rice was analyzed. The results showed no matter the rice Guangjing No.1(O. Sativa L. subsp. japonica) were transformed by p13w4 plasmid carrying anti-sense waxy gene and hygromycin-resistant gene, or in the rice 01Z5202 (O. sativa L. subsp. indica) were co-transformed by p13w8 plasmid carrying anti-sense waxy gene and p1300 plasmid carrying hygromycin-resistant gene, the target gene(s) had been segregated in the progenies; the content of amylose of the transgenic plants was lower than those in non-transgenic ones, and the content of amylose in some of transgenic plants was less than 10.0% (of the weight of grain), which was much lower than those in the control (about 22.04%); and the analysis with SDS-PAGE showed the content of the waxy protein are positively correlated with the con-tent of amylose in the tested transgenic rice materials.
【Fund】: 国家自然科学基金项目(编号:30600049);; 湖南省科技应用基础研究计划重点项目(编号:2007FJ3003);; 湖南省科技计划项目(编号:06FJ3079)资助~~
【CateGory Index】: S511
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