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《Chinese Journal of Sports Medicine》 2009-01
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The Expression of Antigen in Neural Stem Cells Differentiation in vitro

Liu Gang1,Wu Yi1,Jia Jie1,Wang Yang2,Hu Yongshan1,Alastair I H Murchie31 Department of Rehabilitation Medicine,Huashan Hospital,Fudan University,ShangHai,China 2000402 Department of Histology and Embryology,Shanghai Medical School,Fudan University,ShangHai,China 2000323 Drug and Structural Biology Research Institute,Institutes of Biomedical Science,Fudan University,ShangHai,China 200032  
Objective To explore the trends of nestin,SOX2,DCX,Tuj1,GFAP and NeuN expressed in the process of neural stem cells differentiating into neurons in vitro in order to understand the factors affecting the neural stem cells differentiation process in vitro. Methods 12~14 day rat embryo was used as the source of neural stem cells for culture in vitro.Three days after the 1st passage,the neural stem cells were moved into specific culture medium for differentiation.Immunofluorescence methods were used to detect the nestin,SOX2,DCX,Tuj1,GFAP and NeuN at different time points(day1,day4,day7,day10,day14). Results The expression of SOX2 and Nestin of the most cells at the beginning was similar,and then gradually decreased with the passage of time,but the expression of SOX2 lasted relatively longer than nestin.High expression of DCX appeared in day1,day4 and day7,and then gradually decreased;only a few DCX-positive cells in day14 were seen.A small number of Tuj1 positive cells in the day7 appeared,and then followed by increased expression.There was 38.27% Tuj1 positive cells in day14.No NeuN positive cells advented in day1,day4 and day7,while NeuN positive cells accounted for 21.11% in day14.High expression of GFAP-positive cells appeared in day1,and then gradually decreased.The decreasing of expression of GFAP-positive cells was slower than that of nestin,even if there were great number of GFAP-positive cells in day14. Conclusion The expression of specific antigens in the process of differentiation of neural stem cells in vitro is an interlaced process.The decrease in the expression of the undifferentiated cells was consistent with the increase in the expression of the differentiated cells.
【Fund】: 国家高新技术研究发展计划(863计划)(2007AA02Z482);; 复旦大学生物医学研究院开放课题
【CateGory Index】: R329
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