Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Sports Medicine》 2016-01
Add to Favorite Get Latest Update

The Effect of T140 on Type Ⅱ Collagen and Aggrecan in Articular Cartilage of Guinea Pigs

Wang Kun;Zhao Fengkai;Li Yanlin;Jia Di;Yu Yang;Gao Huanyu;Xiao Yu;Li Longteng;Yan Xiangjia;Department of Sports Medicine,First Affiliated Hospital of Kunming Medical University;Department of Osteoarthritis,Central Hospital of Shandong Zaozhuang Mining Group;  
Objective The purpose of this study is to explore the effect of T140(an antagonist of SDF-1/CRCR4 signaling pathway)on type Ⅱ collagen and aggrecan in articular cartilage of guinea pigs. Methods 369-month-old male healthy Hartley guinea pigs were divided into three groups:experimental group(A,n=12),experimental control group(B,n=12) and blank control group(C,n=12). Pigs in group A were implanted Alzet capsule type micro osmotic pressure pump containing T140 diluted by PBS solution and in group B implanted the pump containing PBS solution only. Articular cartilages of knee joint were harvested for measuring m RNA levels of type Ⅱ collagen and aggrecan with RT-PCR and protein levels of type Ⅱ collagen with Western blot12 weeks later. Results The m RNA levels of the type Ⅱ collagen and aggrecan in group A were significantly higher than in groups B and C(P0.05). The content of type Ⅱ collagen in group A was also significantly higher than in groups B and C(P0.05). Conclusion T140 reduces the degradation of type Ⅱ collagen and aggrecan through blocking the SDF-1/CRCR4 signaling pathway and so as decreases the risk of cartilage degeneration.
【Fund】: 国家自然科学基金项目(编号:81460340);; 云南省创新团队项目(编号:2014HC018);; 云南省医学学科带头人培养项目(编号:D-201207)
【CateGory Index】: R684.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved