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《Chinese Journal of Sports Medicine》 2017-01
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Exercise and Diet Modification Delays Liver Cell Apoptosis of Rats with Endoplasmic Reticulum Stress Mediated Nonalcoholic Fatty Liver Disease

Li Junhan;Su Quansheng;Sun Junzhi;Zhang Zhongyang;Chengdu Sport University;Beijing Sport University;  
Objective To explore the effect of exercise and diet modification on liver cell apoptosisof rats with endoplasmic reticulum stress mediated nonalcoholic fatty liver disease(NAFLD). Methods20 of 70 rats were randomly chosen as control group(C,fed with ordinary diet),the remaining 50 were fed with high-fat diet and finally suffered from NAFLD 8 weeks after the experiment,thereafterthey were randomly regrouped into model group(M,n=10), exercise model group(EM,n=10), dietmodification model group(FM,n=10)and exercise combined with diet modification model group(EFM,n=10). Rats in group C were continuously fed with ordinary diet and in groups M and EM werecontinuously fed with high fat diet. The rats in groups FM and EFM was shift from high fat toordinary diet. Rats in groups EM and EFM participated in aerobic swimming for 8 weeks until theend of experiment. Liver pathological changes were observed by oil red O staining and hepatocyteapoptosis was observed by Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling assays.The expression of CCAAT enhancer-binding homologous protein(CHOP),c-Jun n-terminal kinase(JNK)and caspase-12 gene were determined by Western blot,and the expression rate of CHOP and JNK andcaspase-12 by immunohistochemical staining. Results(1)The number of lipid droplets in liver cells ofgroup M significantly increased as compared with group C and the number of lipid droplets in livercells of intervention groups was situated between the number of group C and M.(2)Compared to groupC, the liver cell apoptosis,the protein expressions of CHOP,Caspase12 and JNK gene increased ingroup M. Compared to group M,the liver cell apoptosis and the protein expressions of CHOP andCaspase12 and JNK gene decreased in intervention groups.(3)There was main effect of exercise anddiet on the protein expressions of CHOP and Caspase12 and JNK gene,and they were interacted eachother. Conclusions Endoplasmic reticulum stress mediated liver cell apoptosis participated in thedevelopment of NAFLD,which could be delayed by exercise and diet modification through decreasingthe protein expression of endoplasmic reticulum stress,especially the combination of exercise and dietmodification.
【Fund】: “十二五”国家科技支撑计划重点项目子课题(2012BAK21B01-4);; 四川省科技厅应用基础计划项目(2015JY0155);; 国家体育总局运动医学重点实验室暨运动医学四川省重点实验室资助基金(CTYY2015018)
【CateGory Index】: R575.5
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