Purification and Characteristics of Sakesa Lectin
Cheng Xing et al
Sakesa Lectin was obtained by extraction with PBS, precipitation with ammonium sulfate, and affinity absorption on porcine thyroglobulin-Sepharose 4B from a seed of Phaseolus vulgaris L. SDS-polyacrylamide electrophoresis with or without 1% mercaptoethanol gave one band with molecular weights of 30.26×10~3U, indicating that the lectin consisted of identical subunits. Its total carbohydrate content was 5.0%. The lectin agglutinated rabbit, mouse, chicken and human ABO blood cell, and the activity of this lectin was inhibited by porcine thyroglobulin. Sakesa Lectin was a strong mitogen for human peripheral lymphocytes. The experiments in vitro showed that Sakesa Lectin could agglutinate mouse spleen lymphocytes and Sp2/0 myeloma cells.