Effects of Staphylococcus Aureus on Detection of Specific IgA of Human Cytomegalovirus
Ni Heyu et al
Human Cytomegalovirus (HCMV) IgA antibodies in sera were detected by Enzyme-Linked Immunosorbent Assays (ELISA) after sera were absorbed with staphylococcus aureus. About all of sera and cord sera IgG could be removed when 5% and 6.7% (final concentration) staphylococcus aureus were used respectively. IgA positive rate was also reduced from 15.04% to 3.76% (P0.01) as specific absorption of IgA (at wavelength of 492nm) was reduced in ELISA procedure (P0.01). However no variation of positive rate was found when sera were absorbed with rheumatoid factors absorbent. Therefore, staphylococcal absorption can remove not only CMV IgG but also IgA obviously.