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《Acta Universitis Medicinalis Nahui》 2002-01
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A mutant of rotavirus VP4 gene obtained by megaprimer PCR

He Jinsheng, Wang Jianwei, Dong Jingfang et al (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052)  
Objective To mutate the recognition sequence of Pac I in the Group A Rotavirus KuVP4 gene and obtain nonsense mutant for the application in the novel Ad-Easy adenovirus system. Methods Megaprimer PCR and DNA sequencing were employed. Results The recognition sequence of Pac Ⅰ TTAATTAA was mutated as CTGATCAA. Conclusion Megaprimer PCR is a simple and fast method to obtain mutant.
【Fund】: 国家 8 6 3计划生物和现代农业技术领域资助项目 (编号 :2 0 0 1AA2 15 0 11)
【CateGory Index】: R446.5
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