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《Acta Universitis Medicinalis Nahui》 2002-01
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Establishment and preliminary application of methylation-specific method for detecting p16 gene

Liu Xiaoyin, Wang Huiyuan, Lu Yunxia et al (Anhui Medical University, Hefei 230032)  
Objective To establish a method for analysis of the methylation status of CpG islands of p16 gene:methylation-specific polymerase chain reaction(MSP),and its preliminary application. Methods The denatured target DNA was modified by sodium bisulfate,which converting all unmethylated,but not methylated,cytosines to uracils,and then was amplified with primers specific for methylated versus unmethylated DNA.The 5′CpG islands of p16 gene in leukemia DNA were analyzed by MSP assay. Results There were some methylated 5′CpG islands in p16 of leukemia patients.The methods to denature DNA by heating,to modify the denatured DNA by sodium bisulfate,and to purify the modified DNA by Wizard DNA purification resin were all successful. Conclusion MSP is a simple,sensitive,and specific method for determining the methylation status of virtually any CpG-rich region.
【Fund】: 安徽医科大学自然科学基金 (编号 :5 2 2 0 95、990 7、990 4);; 安徽省自然科学基金 (编号 :990 44312 );; 安徽省教育厅自然科学基金 (编号 :JL97 0 77)
【CateGory Index】: R446.9
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