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《Acta Universitis Medicinalis Nahui》 2002-01
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Construction of expression vector of HIV-1 vpr gene

Guo Li 1, Wang Jianwei 2, Shi Baifen 1 et al ( 1Dept of Microbiology and Parasitology, Anhui Medical University, Hefei 230032; 2Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052)  
Objective To construct hepatocarcinoma specific expression vector of HIV-1 vpr gene. Methods HIV-1 vpr gene segment was amplified from pBSXCYPYVPR-xcTHY with polymerase chain reaction (PCR),and then cloned into pGEM-T vector and the cloning vector pGEM-T/vpr was obtained.Then the vpr gene was recovered and inserted into the upstream of polyA in the pCI vector.Finally The vprA (vpr+polyA) fragment was inserted into the downstream of AFP promoter in the pAF5.1 after it was recovered from the intermedia vector pCI/vpr. Results The hepatocarcinoma specific recombinant expression vector was constructed successfully. Conclusion After the pAFVPRA vector was constructed successfully, the HIV-1 vpr gene can be introduced into the hepatocytes and kill liver cancer cells specifically.
【Fund】: 国家自然科学基金资助项目 (编号 :30 0 0 76 1748)
【CateGory Index】: R373
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【References】
Chinese Journal Full-text Database 1 Hits
1 WEI qiang, LI fan, WANG jian-wei, et al.(Medical school of Jilin University, Changchun 130021 ,China);Construction and identify the recombinant replication-incompetent adenovirous of expressing HIV vpr gene drived by the two AFP promoter[J];中国实验诊断学;2004-02
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Guo Li 1, Wang Jianwei 2, Shi Baifen 1 et al ( 1Dept of Microbiology and Parasitology, Anhui Medical University, Hefei 230032; 2Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052);Construction of expression vector of HIV-1 vpr gene[J];安徽医科大学学报;2002-01
2 YANG Jun *,Steve Yi,WANG Jianwei,et al. * Children's Memorial Institute for Education and Research,USA;Cell cycle G2 arrest,cell death and nuclear localization induced by human immunodeficiency virus type 1 protein R(Vpr) in cervical cancer cells[J];中华实验和临床病毒学杂志;2000-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WEI Qiang~1,WANG Jian-wei~(1*),GUO Li~1,et al.(Institute For Viral Disease Contral and Prevention,China CDC Binjin 100052);Cell cycle G2 arrest and apoptosis induced by adenovirus expressing HIV-1 vpr driven by human alpha-fetoprotein promoter in hepatoma cell lines[J];中国实验诊断学;2006-07
2 Wei Qiang~1, Wang Jianwei~1, Qu Jianguo~1, Zhao Yuqi~2, Hong Tao~1 (~1Institute of Viral Disease Control and Prevention, Center for Disease Control and Prevention of China,Beijing,100052; ~2Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201);Tumor-suppressing effect of recombinant adenovirus encoding HIV-1 Vpr gene driven by human alpha-fetoprotein promoter in nude mice[J];上海医药;2006-07
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