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《Acta Universitis Medicinalis Nahui》 2002-01
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Expression of human telomerase catalytic subunit gene and its protein in gastric cancer

Shao Jinchen, Wu Jifeng (Dept of Pathology, Anhui Medical University, Hefei 230032)  
Objective To investigate the expression of human telomerase catalytic subunit gene and the correlation of its mRNA and protein in gastric cancer. Methods Expression of hTERT mRNA was detected by in situ hybridization(ISH), and expression of hTERT protein was detected by S-P immunohistochemical technique in 53 patients with gastric carcinoma. Results Positive cases of hTERT mRNA were detected in 41(77%) patients with gastric carcinoma, in which 18(82%) were poor differentiated adenocarcinoma, 21(81%) well differentiated adenocarcinoma and 2 mucoid carcinoma(2/5). Positive dying of hTERT protein was detected in 46(87%) patients with gastric carcinoma, in which 20(91%) were poor differentiated adenocarcinoma, 25(96%) well differentiated adenocarcinoma and 1 mucoid carcinoma. Expression of hTERTmRA between poor differentiated adenocarcinoma and well differentiated adenocarcinoma was not significanty different, so was hTERT protein(P0.05). Expression of hTERT mRNA and hTRET protein showed significant positive correlation(r s=0.623, P0.001). Conclusion The dectetion of hTERT gene expression may provide a new morphological method for diagnosis and distinctive diagnosis of gastric cancer.
【Fund】: 安徽省教育厅科研基金 (编号 :99j10 0 91)
【CateGory Index】: R735.2
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1 Xi Xian Yao~1, Lei Yin~1, Jie Ying Zhang~2, Wen Yuan Bai~1, Ying Min Li~3 and Zhong Cheng Sun~4 1 The Department of Digestive Medicine, and 2 Department of Pathology, the 2nd Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China 3 The Department of Pathology, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China 4 The Traditional Chinese Medical College of Hebei Medical University, Shijiazhuang 050091, Hebei Province, China;hTERT expression and cellular immunity in gastric cancer and precancerosis[J];World Chinese Journal of Digestology;2001-05
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1 ZHANG Hong-bo, WEI Qi-you. Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha 410011, China;Expression of hTRT and Bax in Papillary Thyroid Carcinoma[J];Journal of Chinese Physician;2005-05
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