Effects of rhIL-10 on peripheral blood mononuclear cells of psoriasis patients and normal person
Chen Feihu, Chen Minzhu, Li Jun et al(Dept of pharmacy, Anhui Medical University, Hefei 230032)
Objective To observe the effects of recombinant human i nterleukin 10 (rhIL 10 ) on peripheral blood mononuclear cells (PBMC) of psoriasis patients and normal persons. Methods PHA induced PBMC production of IL 2 and IFN r, LPS induced production of IL 1 , IL 6, IL 8 and nitric oxide (NO) were determined. Results It was revealed that the concentrations of IFN γ, IL 1, IL 6 and IL 8 produced by activated PBMC of psoriasis patients were significantly higher than that of normal persons in the absence of rhIL 10. Similarly, IL 2 and NO level were also higher in the former group than the latter one. These data suggest that the selected patients were in active stage of psoriasis. Increased concentrations of rhIL 10 significantly inhibited production of cytokines (IL 2 and IFN γ, IL 1, IL 6, IL 8 and NO) by PBMC in the presence of PHA or LPS in a dose dependent way. There was not any difference ( P 0 05) between the two groups, indicating their sensitivities to rhIL 10 were similar with each other. Whether rhIL 10 existed or not, anti IL 10MAB (2 5, 5, 10, 20, 40 mg/L) restored production of IL 2 and IFN γ by PHA induced PBMC of both groups. However, the recovery of cytokines production of psoriasis PBMC was less than that of normal people. These results indicated that anti IL 10MAB was able to recognize rhIL 10, as well as natural IL 10 (a negative factor) and the productivity of IL 10 in patient PBMC was impaired. Conclusion RhIL 10 can significantly inhibit the pro inflammatory cytokine production (IL 1, IL 6, IL 8 and NO) as well as the immunological cytokine production (IL 2 and IFNr), suggesting that rhIL 10 has combined immunosuppressive and anti inflammatory activity.