Determination of serum manganese with graphite AAS
Yu Qiuyue, Wang Xianliang, Yang Yongjian et al(School of Public Health, Anhui Medical University, Hefei 230032)
Objective To set up a new method of detecting the l evel of manganese in serum with the graphite atomic absorption spectroscopy(AAS) . Methods The serum was diluted with HNO 3 and centrifuged 10 minu tes by 15 000 r/min first and the solution above in the tube was going to be determined. Results The optimal temperatures for cineration, atomi zation and clearance were 1 300 ℃, 1 900 ℃ and 2 450 ℃ with the matri x modifier of a unit of 5 μg Pd and 3 μg Mg(NO 3) 2, respectively. The avera ge reclamation rate was 99 56% and the coefficient of variation was about 5%; t he sensitivity was 6 376 10 pg / 0 004 4 A and the minimal level could be detected was 6 7 pg. Conclusion This is a practicable and cre dible method for detection serum Mn with AAS.