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Chemical Constituents from n-BuOH Extract of Codonopsis pilosula

ZHUANG Xiaocui;YAN Yongming;ZHANG Qingzhi;CHENG Yongxian;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences;College of Traditional Chinese Medicine,Yunnan University of Traditional Chinese Medicine;  
Objective: To study the chemical constituents from the n-butanol extract of Codonopsis pilosula produced in Yunnan province.Methods: The chemical constituents were isolated by various column chromatography.Their structures were identified by NMR and MS.Results: Twenty-one known compounds were isolated from n-butanol extract of C.pilosula from in Yunnan province.Their structures were identified as 7 R,8 R-threo-4,7,9,9'-tetrahydroxy-3-methoxy-8-O-4'-neolignan-3'-O-β-D-glucopyranoside(1),catechin-7-O-β-D-glucopyranside(2),syringin(3),coniferin(4),tangshenoside II(5),(Z)-4-hydroxycinnamyl alcohol 4-O-β-D-glucopyranoside(6),2-hydroxymethylphenyl-1-O-β-D-glucopyranoside(7),benzyl-6-O-β-D-xylopyranosyl-O-β-D-glucopyranoside(8),(2 E)-2-(β-D-glucopyranosyloxy)-3-phenyl-2-propenoic acid(9),(2 Z)-2-(β-D-glucopyranosyloxy)-3-phenyl-2-propenoic acid(10),2-phenylethyl 6-O-β-D-xylopyranosyl-O-β-D-glucopyranoside(11),tangsheno side I(12),3-hydroxy-2-{4-[(1 E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenoxy} propyl-β-D-glucopyranoside(13),(2 E)-4-hydroxy-2-hexenoic acid(14),(2 E)-3-methyl-2-pentenedioic acid(15),5-acetylshikimic acid(16),tryptophan(17),adenosine(18),niacin(19),caffeine(20),and 5-(methoxycarbonyl)-2-pyrrolidone(21),respectively,by spectroscopic methods.Conclusion: Among these substances,compounds 8,9,11,13 and21 were isolated from C.pilosula for the first time.
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